Boster Immunoleader

ELISA/assay

Human IL-10 PicoKine ELISA Kit

EK0416

1 x96 well plate

250 USD

Human IL-10 PicoKine ELISA Kit

EK0416

250 USD

Sandwich ELISA kit for Quantitative Detection of Human IL-10

E.coli, S19-N178

1 x96 well plate

7.8pg/ml-500pg/ml(cellculturesupernates)3.4pg/ml-250pg/ml(humanserum,plasma)

<0.5pg/ml

Sample 1: n=16; Mean(pg/ml): 25; Standard deviation: 1.35; CV(%): 5.4 Sample 2: n=16; Mean(pg/ml): 112; Standard deviation: 5.38; CV(%): 4.8 Sample 3: n=16; Mean(pg/ml): 280; Standard deviation: 8.68; CV(%): 3.1

Sample 1: n=24; Mean(pg/ml): 34; Standard deviation: 2.55; CV(%): 7.5 Sample 2: n=24; Mean(pg/ml): 184; Standard deviation: 14.54; CV(%): 7.9 Sample 3: n=24; Mean(pg/ml): 312; Standard deviation: 20.9; CV(%): 6.7

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human IL-10 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human IL-10 antibody 1 ST0000-10 Lyophilized recombinant human IL-10 standard 10ng/tubex2 AR1107 Biotinylated anti- human IL-10 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.

IL10

Interleukin-10

interleukin 10

CSIF Cytokine synthesis inhibitory factor GVHDS IL 10 IL-10 IL10 IL10_HUMAN IL10A Interleukin 10 Interleukin-10 Interleukin10 MGC126450 MGC126451 T cell growth inhibitory factor TGIF

P22301