Boster Immunoleader

ELISA/assay

Human IL-8 PicoKine ELISA Kit

EK0413

1 x96 well plate

250 USD

Human IL-8 PicoKine ELISA Kit

EK0413

250 USD

Sandwich ELISA kit for Quantitative Detection of Human IL-8

E.coli, S28-S99

1 x96 well plate

15.6pg/ml-1000pg/ml(cellculturesupernates)7.8pg/ml-500pg/ml(serum,plasma)

<1pg/ml

Sample 1: n=16; Mean(pg/ml): 115; Standard deviation: 6.67; CV(%): 5.8 Sample 2: n=16; Mean(pg/ml): 357; Standard deviation: 17.5; CV(%): 4.9 Sample 3: n=16; Mean(pg/ml): 616; Standard deviation: 41.3; CV(%): 6.7

Sample 1: n=24; Mean(pg/ml): 176; Standard deviation: 13.38; CV(%): 7.6 Sample 2: n=24; Mean(pg/ml): 392; Standard deviation: 21.17; CV(%): 5.4 Sample 3: n=24; Mean(pg/ml): 649; Standard deviation: 45.43; CV(%): 7

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human IL-8 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human IL-8 antibody 1 ST0000-10 Lyophilized recombinant human IL-8 standard 10ng/tubex2 AR1107 Biotinylated anti- human IL-8 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor(MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members(platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.

CXCL8

Interleukin-8

chemokine (C-X-C motif) ligand 8

(Ala-IL-8)77 (Ser-IL-8)72 3 10C 3-10C AMCF I AMCF-I AMCFI b ENAP B-ENAP Beta-thromboglobulin-like protein C X C motif chemokine 8 C-X-C motif chemokine 8 Chemokine (C X C motif) ligand 8 CXCL8 Emoctakin GCP 1 GCP-1 GCP/IL-8 protein I GCP/IL-8 protein II GCP/IL-8 protein III GCP/IL-8 protein IV GCP/IL-8 protein V GCP/IL-8 protein VI GCP1 Granulocyte chemotactic protein 1 IL 8 IL-8 IL-8(1-77) IL-8(9-77) IL8 IL8/NAP1 form I IL8/NAP1 form II IL8/NAP1 form III IL8/NAP1 form IV IL8/NAP1 form V IL8/NAP1 form VI IL8_HUMAN Interleukin 8 K60 LECT LUCT Lymphocyte derived neutrophil activating factor Lymphocyte-derived neutrophil-activating factor LYNAP MDNCF MDNCF-b MDNCF-c MONAP Monocyte derived neutrophil activating peptide Monocyte derived neutrophil chemotactic factor Monocyte-derived neutrophil chemotactic factor Monocyte-derived neutrophil-activating peptide NAF NAP 1 NAP-1 NAP1 Neutrophil activating peptide 1 Neutrophil activating protein 1 Neutrophil-activating factor Neutrophil-activating protein 1 Protein 3 10C Protein 3-10C SCYB8 Small inducible cytokine subfamily B member 8 T cell chemotactic factor T-cell chemotactic factor TSG 1 TSG-1 TSG1

P10145