Boster Immunoleader

ELISA/assay

Rat IL-4 PicoKine ELISA Kit

EK0406

1 x96 well plate

320 USD

Rat IL-4 PicoKine ELISA Kit

EK0406

320 USD

Sandwich ELISA kit for Quantitative Detection of Rat IL-4

E.coli, C25-S147

1 x96 well plate

15.6pg/ml-1000pg/ml

<1pg/ml

Sample 1: n=16; Mean(pg/ml): 54.7; Standard deviation: 3.23; CV(%): 5.9 Sample 2: n=16; Mean(pg/ml): 428; Standard deviation: 21.83; CV(%): 5.1 Sample 3: n=16; Mean(pg/ml): 738; Standard deviation: 31.73; CV(%): 4.3

Sample 1: n=24; Mean(pg/ml): 53.8; Standard deviation: 4.79; CV(%): 8.9 Sample 2: n=24; Mean(pg/ml): 387; Standard deviation: 25.93; CV(%): 6.7 Sample 3: n=24; Mean(pg/ml): 729; Standard deviation: 45.2; CV(%): 6.2

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of rat IL-4 in cell culture supernates and serum.

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat IL-4 antibody 1 ST0000-10 Lyophilized recombinant rat IL-4 standard 10ng/tubex2 AR1107 Biotinylated anti- rat IL-4 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. Rat IL-4 is a 13.5KDa glycoprotein, consisting of 147 amino acids in precursor form and 123 amino acids in mature form.

IL4

Interleukin-4

interleukin 4

B cell growth factor 1 B cell stimulatory factor 1 BCGF 1 BCGF1 Binetrakin BSF 1 BSF1 IL 4 Interleukin 4 Lymphocyte stimulatory factor 1 MGC79402 Pitrakinra

P20096