Boster Immunoleader

ELISA/assay

Human IL-4 PicoKine ELISA Kit

EK0404

1 x96 well plate

250 USD

Human IL-4 PicoKine ELISA Kit

EK0404

250 USD

Sandwich ELISA kit for Quantitative Detection of Human IL-4

E.coli, H25-S153

1 x96 well plate

15.6pg/ml-1000pg/ml

<1.5pg/ml

Sample 1: n=16; Mean(pg/ml): 55.4; Standard deviation: 2.5; CV(%): 4.5 Sample 2: n=16; Mean(pg/ml): 361; Standard deviation: 14.8; CV(%): 4.1 Sample 3: n=16; Mean(pg/ml): 638; Standard deviation: 37.64; CV(%): 5.9

Sample 1: n=24; Mean(pg/ml): 45.8; Standard deviation: 2.977; CV(%): 6.5 Sample 2: n=24; Mean(pg/ml): 375; Standard deviation: 24; CV(%): 6.4 Sample 3: n=24; Mean(pg/ml): 656; Standard deviation: 46.58; CV(%): 7.1

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human IL-4 in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human IL-4 antibody 1 ST0000-10 Lyophilized recombinant human IL-4 standard 10ng/tubex2 AR1107 Biotinylated anti- human IL-4 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.

IL4

Interleukin-4

interleukin 4

B cell growth factor 1 B cell stimulatory factor 1 BCGF 1 BCGF1 Binetrakin BSF 1 BSF1 IL 4 Interleukin 4 Lymphocyte stimulatory factor 1 MGC79402 Pitrakinra

D4HNR6