Boster Immunoleader

ELISA/assay

Human CD25/IL-2sR alpha PicoKine ELISA Kit

EK0400

1 x96 well plate

369 USD

Human CD25/IL-2sR alpha PicoKine ELISA Kit

EK0400

369 USD

Sandwich ELISA kit for Quantitative Detection of Human CD25/IL-2sR alpha

NSO,E22-C213

1 x96 well plate

78pg/ml-5000pg/ml

<5pg/ml

Sample 1: n=16; Mean(ng/ml): 0.22; Standard deviation: 0.014; CV(%): 6.3 Sample 2: n=16; Mean(ng/ml): 0.63; Standard deviation: 0.041; CV(%): 6.5 Sample 3: n=16; Mean(ng/ml): 2.53; Standard deviation: 0.132; CV(%): 5.2

Sample 1: n=24; Mean(ng/ml): 0.25; Standard deviation: 0.020; CV(%): 8 Sample 2: n=24; Mean(ng/ml): 0.71; Standard deviation: 0.052; CV(%): 7.3 Sample 3: n=24; Mean(ng/ml): 2.36; Standard deviation: 0.191; CV(%): 8.1

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human CD25 in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human CD25/IL-2sR alpha antibody 1 ST0000-10 Lyophilized recombinant human CD25/IL-2sR alpha standard 10ng/tubex2 AR1107 Biotinylated anti- human CD25/IL-2sR alpha antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

IL2RA

Interleukin-2 receptor subunit alpha

interleukin 2 receptor, alpha

CD122 CD122 antigen CD132 CD132 antigen CD25 CD25 antigen Cytokine receptor common gamma chain Gamma C High affinity IL 2 receptor subunit beta IDDM10 IL 2 RA IL 2 receptor IL 2 receptor alpha subunit IL 2R gamma chain IL2 RA IL2R IL2RA IL2RB IL2RG IMD4 interleukin 2 receptor alpha Interleukin 2 receptor alpha chain interleukin 2 receptor beta Interleukin 2 receptor beta chain interleukin 2 receptor gamma Interleukin 2 receptor gamma (severe combined immunodeficiency) Interleukin 2 receptor gamma chain p55 p64 P70 75 p75 SCIDX SCIDX1 TAC antigen TCGFR XSCID

P01589