Boster Immunoleader

ELISA/assay

Human IGFBP-3 PicoKine ELISA Kit

EK0386

1 x96 well plate

350 USD

Human IGFBP-3 PicoKine ELISA Kit

EK0386

350 USD

Sandwich ELISA kit for Quantitative Detection of Human IGFBP-3

NSO, A28Q-291

1 x96 well plate

156.2pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 1.12; Standard deviation: 0.058; CV(%): 5.2 Sample 2: n=16; Mean(ng/ml): 3.41; Standard deviation: 0.198; CV(%): 5.8 Sample 3: n=16; Mean(ng/ml): 5.23; Standard deviation: 0.241; CV(%): 4.6

Sample 1: n=24; Mean(ng/ml): 1.24; Standard deviation: 0.077; CV(%): 6.2 Sample 2: n=24; Mean(ng/ml): 3.26; Standard deviation: 0.231; CV(%): 7.1 Sample 3: n=24; Mean(ng/ml): 6.34; Standard deviation: 0.552; CV(%): 8.7

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human IGFBP-3 in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human IGFBP-3 antibody 1 ST0000-10 Lyophilized recombinant human IGFBP-3 standard 10ng/tubex2 AR1107 Biotinylated anti- human IGFBP-3 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Insulin-like growth factor(IGF)-binding protein-3(IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene(IGFBP3) expression. Insulin-like growth factor-binding protein(IGFBP)-3, well characterized as the carrier of insulin-like growth factor(IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix. IGFBP3 is also located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-3, consisting of 265 amino acids with the molecular mass of 29kDa. As a result of glycosylation, the molecular mass is 41KDa.

IGFBP3

Insulin-like growth factor-binding protein 3

insulin-like growth factor binding protein 3

Acid stable subunit of the 140 K IGF complex Binding protein 29 Binding protein 53 BP 53 BP53 Growth hormone dependent binding protein IBP 3 IBP3 IGF binding protein 3 IGFBP 3 Insulin Like Growth Factor Binding Protein 3

B3KPF0