Boster Immunoleader

ELISA/assay

Mouse IGF-1 PicoKine ELISA Kit

EK0378

1 x96 well plate

320 USD

Mouse IGF-1 PicoKine ELISA Kit

EK0378

320 USD

Sandwich ELISA kit for Quantitative Detection of Mouse IGF-1

E.coli, G49-A118

1 x96 well plate

62.5pg/ml-4000pg/ml

<5pg/ml

Sample 1: n=16; Mean(pg/ml): 447; Standard deviation: 28.61; CV(%): 6.4 Sample 2: n=16; Mean(pg/ml): 1253; Standard deviation: 53.88; CV(%): 4.3 Sample 3: n=16; Mean(pg/ml): 2346; Standard deviation: 115; CV(%): 4.9

Sample 1: n=24; Mean(pg/ml): 450; Standard deviation: 39.15; CV(%): 8.7 Sample 2: n=24; Mean(pg/ml): 1238; Standard deviation: 64.38; CV(%): 5.2 Sample 3: n=24; Mean(pg/ml): 2625; Standard deviation: 144.4; CV(%): 5.5

There is no detectable cross-reactivity with IGF-2.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse IGF-1 in cell culture supernates, serum and plasma(heparin, EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse IGF-1 antibody 1 ST0000-10 Lyophilized recombinant mouse IGF-1 standard 10ng/tubex2 AR1107 Biotinylated anti- mouse IGF-1 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Insulin-like growth factor 1(IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD.1 The IGF1 gene, mapped on 12q22-q24. contains 5 exons. Exons 1-4 encode the 195-amino acid precursor(IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide(IGF1A). The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98% of IGF-1 is always bound to one of 6 binding proteins(IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.

IGF1

Insulin-like growth factor I

insulin-like growth factor 1 (somatomedin C)

IBP1 IGF 1 IGF I IGF IA IGF IB IGF-I Igf1 IGF1_HUMAN IGF1A IGFI Insulin like growth factor 1 (somatomedin C) Insulin like growth factor IA Insulin like growth factor IB Insulin-like growth factor I Mechano growth factor MGF Somatomedia C Somatomedin C Somatomedin-C

E9PU89