Boster Immunoleader

ELISA/assay

Mouse ICAM-1 PicoKine ELISA Kit

EK0371

1 x96 well plate

400 USD

Mouse ICAM-1 PicoKine ELISA Kit

EK0371

400 USD

Sandwich ELISA kit for Quantitative Detection of Mouse ICAM-1

NSO, Q28-N485

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 1046; Standard deviation: 47.07; CV(%): 4.5 Sample 2: n=16; Mean(pg/ml): 3779; Standard deviation: 215.4; CV(%): 5.7 Sample 3: n=16; Mean(pg/ml): 6637; Standard deviation: 404.9; CV(%): 6.1

Sample 1: n=24; Mean(pg/ml): 1145; Standard deviation: 61.83; CV(%): 5.4 Sample 2: n=24; Mean(pg/ml): 4476; Standard deviation: 282; CV(%): 6.3 Sample 3: n=24; Mean(pg/ml): 6450; Standard deviation: 464.4; CV(%): 7.2

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse ICAM-1 in cell culture supernates, serum, plasma(heparin, EDTA) and tissue homogenates.

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse ICAM-1 antibody 1 ST0000-10 Lyophilized recombinant mouse ICAM-1 standard 10ng/tubex2 AR1107 Biotinylated anti- mouse ICAM-1 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Intercellular adhesion molecule-1(ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated(LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1(ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen(LFA-1) or macrophage-1 antigen(Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses. The standard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds. The single chain is Gln28-Asn 485. It has 457 amino acids with the molecular mass of 76.8kDa. As a result of glycosylation, the molecular mass of 110-130KDa is revealed by SDS-PAGE.

ICAM1

Intercellular adhesion molecule 1

intercellular adhesion molecule 1

BB2 CD 54 CD54 CD54 antigen Cell surface glycoprotein P3.58 Human rhinovirus receptor ICAM 1 Intercellular adhesion molecule 1 Major group rhinovirus receptor MALA2 MGC6195 MyD10 P3.58 Surface antigen of activated B cells

P13597