Boster Immunoleader

ELISA/assay

Human GM-CSF PicoKine ELISA Kit

EK0364

1 x96 well plate

400 USD

Human GM-CSF PicoKine ELISA Kit

EK0364

400 USD

Sandwich ELISA kit for Quantitative Detection of Human GM-CSF

E.coli, A18-E144

1 x96 well plate

15.6pg/ml-1000pg/ml

<1pg/ml

Sample 1: n=16; Mean(pg/ml): 104; Standard deviation: 4.58; CV(%): 4.4 Sample 2: n=16; Mean(pg/ml): 485; Standard deviation: 15.52; CV(%): 3.2 Sample 3: n=16; Mean(pg/ml): 621; Standard deviation: 27.95; CV(%): 4.5

Sample 1: n=24; Mean(pg/ml): 107; Standard deviation: 6.53; CV(%): 6.1 Sample 2: n=24; Mean(pg/ml): 463; Standard deviation: 29.63; CV(%): 6.4 Sample 3: n=24; Mean(pg/ml): 618; Standard deviation: 35.23; CV(%): 5.7

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human GM-CSF in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human GM-CSF antibody 1 ST0000-10 Lyophilized recombinant human GM-CSF standard 10ng/tubex2 AR1107 Biotinylated anti- human GM-CSF antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Granulocyte-macrophage colony-stimulating factor(GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.

CSF2

Granulocyte-macrophage colony-stimulating factor

colony stimulating factor 2 (granulocyte-macrophage)

Colony stimulating factor Colony stimulating factor 2 (granulocyte macrophage) CSF CSF 2 CSF2 GMCSF Granulocyte macrophage colony stimulating factor MGC131935 MGC138897 Molgramostin Sargramostim

P04141