Boster Immunoleader

ELISA/assay

Rat GDNF PicoKine ELISA Kit

EK0363

1 x96 well plate

380 USD

Rat GDNF PicoKine ELISA Kit

EK0363

380 USD

Sandwich ELISA kit for Quantitative Detection of Rat GDNF

sf21, S78-I211

1 x96 well plate

31.2pg/ml-2000pg/ml

<4pg/ml

Sample 1: n=16; Mean(pg/ml): 155; Standard deviation: 6.98; CV(%): 4.5 Sample 2: n=16; Mean(pg/ml): 624; Standard deviation: 26.21; CV(%): 4.2 Sample 3: n=16; Mean(pg/ml): 1316; Standard deviation: 72.38; CV(%): 5.5

Sample 1: n=24; Mean(pg/ml): 148; Standard deviation: 10.06; CV(%): 6.8 Sample 2: n=24; Mean(pg/ml): 747; Standard deviation: 59.01; CV(%): 7.9 Sample 3: n=24; Mean(pg/ml): 1324; Standard deviation: 102; CV(%): 7.7

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of rat GDNF in cell culture supernates, serum and plasma(heparin, EDTA).

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat GDNF antibody 1 ST0000-10 Lyophilized recombinant rat GDNF standard 10ng/tubex2 AR1107 Biotinylated anti- rat GDNF antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Glial cell line-derived neurotrophic factor(GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures. The standard product used in this kit is recombinant rat GDNF, which is a dimmer composed of two chains with 134 amino acids.

GDNF

Glial cell line-derived neurotrophic factor

glial cell derived neurotrophic factor

Astrocyte derived trophic factor ATF ATF1 ATF2 Glial cell line derived neurotrophic factor HFB1 GDNF hGDNF HSCR3

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