Boster Immunoleader

ELISA/assay

Mouse G-CSF PicoKine ELISA Kit

EK0361

1 x96 well plate

380 USD

Mouse G-CSF PicoKine ELISA Kit

EK0361

380 USD

Sandwich ELISA kit for Quantitative Detection of Mouse G-CSF

E.coli, V31-A208

1 x96 well plate

31.2pg/ml-2000pg/ml

<4pg/ml

Sample 1: n=16; Mean(pg/ml): 155; Standard deviation: 4.1; CV(%): 4.1 Sample 2: n=16; Mean(pg/ml): 737; Standard deviation: 5.2; CV(%): 5.2 Sample 3: n=16; Mean(pg/ml): 1426; Standard deviation: 6.3; CV(%): 6.3

Sample 1: n=24; Mean(pg/ml): 147; Standard deviation: 9.114; CV(%): 6.2 Sample 2: n=24; Mean(pg/ml): 607; Standard deviation: 49.77; CV(%): 8.2 Sample 3: n=24; Mean(pg/ml): 1524; Standard deviation: 129.54; CV(%): 8.5

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of Mouse G-csf in cell culture supernates, serum and plasma(heparin, EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse G-CSF antibody 1 ST0000-10 Lyophilized recombinant mouse G-CSF standard 10ng/tubex2 AR1107 Biotinylated anti- mouse G-CSF antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Granulocyte colony-stimulating factor(G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17.3 The standard product used in this kit is recombinant Mouse G-csf, consisting of 179 amino acids with the molecular mass of 19KDa.

CSF3

Granulocyte colony-stimulating factor

colony stimulating factor 3 (granulocyte)

C17orf33 Colony stimulating factor 3 (granulocyte) CSF 3 CSF beta CSF3 CSF3OS Csfg Filgrastim GCSA GCSF Granulocyte colony stimulating fact Lenograstim Macrophage granulocyte inducer 2 MGC45931 MGI 2 MGI IG OTTMUSP00000006785 Pluripoietin RP23-395E10.4

P09920