Boster Immunoleader

ELISA/assay

Human Fractalkine/CX3CL1 PicoKine ELISA Kit

EK0356

1 x96 well plate

400 USD

Human Fractalkine/CX3CL1 PicoKine ELISA Kit

EK0356

400 USD

Sandwich ELISA kit for Quantitative Detection of Human Fractalkine/CX3CL1

NSO, Q25-R339

1 x96 well plate

156pg/ml-10,000pg/ml

<50pg/ml

Sample 1: n=16; Mean(ng/ml): 1.5; Standard deviation: 0.063; CV(%): 4.2 Sample 2: n=16; Mean(ng/ml): 3.8; Standard deviation: 0.122; CV(%): 3.2 Sample 3: n=16; Mean(ng/ml): 6.6; Standard deviation: 0.31; CV(%): 4.7

Sample 1: n=24; Mean(ng/ml): 1.3; Standard deviation: 0.111; CV(%): 8.5 Sample 2: n=24; Mean(ng/ml): 3.7; Standard deviation: 0.237; CV(%): 6.4 Sample 3: n=24; Mean(ng/ml): 6.5; Standard deviation: 0.403; CV(%): 6.2

There is detectable cross-reactivity with Eotaxin, MCP-1, MCP-2, 6Ckine, MARC< 0.2%.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human Fractalkine in cell culture supernates, serum and plasma(heparin, EDTA), saliva, urine and human milk.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human Fractalkine/CX3CL1 antibody 1 ST0000-10 Lyophilized recombinant human Fractalkine/CX3CL1 tandard 10ng/tubex2 AR1107 Biotinylated anti- human Fractalkine/CX3CL1 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Chemokine(C-X3-C motif) ligand 1(CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine(in humans) and neurotactin(in mice). CX3CL1 is produced as a long protein(with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble(90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22. It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.

CX3CL1

Fractalkine

chemokine (C-X3-C motif) ligand 1

A 152E5.2 AB030188 ABCD 3 ABCD3 C X3 C motif chemokine 1 C3Xkine Chemokine (C X3 C motif) ligand 1 Chemokine C X3 C motif ligand 1 Chemokine CX3C Motif Ligand 1 CX3C membrane anchored chemokine CXC3 CXC3C FKN Fractalkine Neurotactin NTN NTT SCYD 1 SCYD1 Small inducible cytokine D1 Small inducible cytokine subfamily D (Cys X3 Cys), member 1 Small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin) Small inducible cytokine subfamily D member 1

A0N0N7