Boster Immunoleader

ELISA/assay

Mouse Fibronectin PicoKine ELISA Kit

EK0351

1 x96 well plate

250 USD

Mouse Fibronectin PicoKine ELISA Kit

EK0351

250 USD

Sandwich ELISA kit for Quantitative Detection of Mouse Fibronectin

from plasma

1 x96 well plate

156pg/ml-10,000pg/ml

<15pg/ml

Sample 1: n=16; Mean(ng/ml): 1.56; Standard deviation: 0.064; CV(%): 4.1 Sample 2: n=16; Mean(ng/ml): 3.24; Standard deviation: 0.136; CV(%): 4.2 Sample 3: n=16; Mean(ng/ml): 5.13; Standard deviation: 0.267; CV(%): 5.2

Sample 1: n=24; Mean(ng/ml): 1.34; Standard deviation: 0.091; CV(%): 6.8 Sample 2: n=24; Mean(ng/ml): 3.29; Standard deviation: 0.243; CV(%): 7.4 Sample 3: n=24; Mean(ng/ml): 5.28; Standard deviation: 0.396; CV(%): 7.5

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse Fibronectin in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse Fibronectin antibody 1 ST0000-10 Lyophilized natural mouse Fibronectin standard 10ng/tubex2 AR1107 Biotinylated anti- mouse Fibronectin antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Fibronectin(FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.

FN1

Fibronectin

fibronectin 1

CIG Cold insoluble globulin Cold-insoluble globulin Fibronectin 1 FINC FINC_HUMAN FN FN1 FNZ Migration stimulating factor MSF Ugl-Y3

P09055