Boster Immunoleader

ELISA/assay

Human FASL PicoKine ELISA Kit

EK0337

1 x96 well plate

420 USD

Human FASL PicoKine ELISA Kit

EK0337

420 USD

Sandwich ELISA kit for Quantitative Detection of Human FASL

CHO, P134-L281

1 x96 well plate

15.6pg/ml-1000pg/ml

<2pg/ml

Sample 1: n=16; Mean(pg/ml): 108; Standard deviation: 5.62; CV(%): 5.2 Sample 2: n=16; Mean(pg/ml): 257; Standard deviation: 12.59; CV(%): 4.9 Sample 3: n=16; Mean(pg/ml): 619; Standard deviation: 36.52; CV(%): 5.9

Sample 1: n=24; Mean(pg/ml): 112; Standard deviation: 9.74; CV(%): 8.7 Sample 2: n=24; Mean(pg/ml): 249; Standard deviation: 22.66; CV(%): 9.1 Sample 3: n=24; Mean(pg/ml): 623; Standard deviation: 44.86; CV(%): 7.2

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human FASL in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human FASL antibody 1 ST0000-10 Lyophilized recombinant human FASL standard 10ng/tubex2 AR1107 Biotinylated anti- human FASL antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Fas ligand(FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor(TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.

FASLG

Tumor necrosis factor ligand superfamily member 6

Fas ligand (TNF superfamily, member 6)

Apoptosis (APO 1) antigen ligand 1 Apoptosis antigen ligand APT1LG1 APTL CD178 CD95 L CD95 ligand CD95L CD95L protein Fas antigen ligand FASL FASLG TNF superfamily member 6 TNFSF6 Tumor necrosis factor (ligand) superfamily member 6 Tumor necrosis factor ligand superfamily member 6

Q99PH8