Boster Immunoleader

ELISA/assay

Mouse FAS PicoKine ELISA Kit

EK0336

1 x96 well plate

300 USD

Mouse FAS PicoKine ELISA Kit

EK0336

300 USD

Sandwich ELISA kit for Quantitative Detection of Mouse Soluble FAS

sf21, Q22-R169

1 x96 well plate

31.2pg/ml-2000pg/ml

<3pg/ml

Sample 1: n=16; Mean(pg/ml): 107; Standard deviation: 9.52; CV(%): 8.9 Sample 2: n=16; Mean(pg/ml): 728; Standard deviation: 30.58; CV(%): 4.2 Sample 3: n=16; Mean(pg/ml): 1407; Standard deviation: 54.87; CV(%): 3.9

Sample 1: n=24; Mean(pg/ml): 116; Standard deviation: 10.56; CV(%): 9.1 Sample 2: n=24; Mean(pg/ml): 739; Standard deviation: 40.65; CV(%): 5.5 Sample 3: n=24; Mean(pg/ml): 1412; Standard deviation: 80.48; CV(%): 5.7

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse FAS in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse sFAS antibody 1 ST0000-10 Lyophilized recombinant mouse sFAS standard 10ng/tubex2 AR1107 Biotinylated anti- mouse sFAS antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor(NGF)/tumour necrosis factor(TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48-kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo.

FAS

Tumor necrosis factor receptor superfamily member 6

Fas cell surface death receptor

ALPS1A APO 1 APO 1 cell surface antigen Apo-1 antigen APO1 Apo1 antigen Apoptosis antigen 1 Apoptosis mediating surface antigen FAS Apoptosis-mediating surface antigen FAS APT1 CD95 CD95 antigen Fas Fas AMA Fas antigen FAS1 FASLG receptor FASTM TNF receptor superfamily member 6 TNFRSF6 TNR6_HUMAN Tumor necrosis factor receptor superfamily member 6

Q8C350