Boster Immunoleader

ELISA/assay

Human sFAS PicoKine ELISA Kit

EK0335

1 x96 well plate

300 USD

Human sFAS PicoKine ELISA Kit

EK0335

300 USD

Sandwich ELISA kit for Quantitative Detection of Human Soluble FAS

NSO, R17-N173

1 x96 well plate

31.2pg/ml-2000pg/ml

<3pg/ml

Sample 1: n=16; Mean(pg/ml): 138; Standard deviation: 5.80; CV(%): 4.2 Sample 2: n=16; Mean(pg/ml): 828; Standard deviation: 25.67; CV(%): 3.1 Sample 3: n=16; Mean(pg/ml): 1507; Standard deviation: 70.83; CV(%): 4.7

Sample 1: n=24; Mean(pg/ml): 146; Standard deviation: 10.95; CV(%): 7.5 Sample 2: n=24; Mean(pg/ml): 839; Standard deviation: 45.31; CV(%): 5.4 Sample 3: n=24; Mean(pg/ml): 1512; Standard deviation: 83.16; CV(%): 5.5

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human FAS in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human sFAS antibody 1 ST0000-10 Lyophilized recombinant human sFAS standard 10ng/tubex2 AR1107 Biotinylated anti- human sFAS antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor(NGF)/tumour necrosis factor(TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48-kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo. The FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis.

FAS

Tumor necrosis factor receptor superfamily member 6

Fas cell surface death receptor

ALPS1A APO 1 APO 1 cell surface antigen Apo-1 antigen APO1 Apo1 antigen Apoptosis antigen 1 Apoptosis mediating surface antigen FAS Apoptosis-mediating surface antigen FAS APT1 CD95 CD95 antigen Fas Fas AMA Fas antigen FAS1 FASLG receptor FASTM TNF receptor superfamily member 6 TNFRSF6 TNR6_HUMAN Tumor necrosis factor receptor superfamily member 6

P25445