product summary
request information :
company name :
Boster Immunoleader
product type :
ELISA/assay
product name :
Human Eotaxin PicoKine ELISA Kit
catalog :
EK0329
quantity :
1 x96 well plate
price :
320 USD
product information
Product Name :
Human Eotaxin PicoKine ELISA Kit
Catalog Number :
EK0329
Price :
320 USD
Description short :
Sandwich ELISA kit for Quantitative Detection of Human Eotaxin
immunogen :
E.coli, G24-P97
Pack Size :
1 x96 well plate
Assay Range :
15.6pg/ml-1000pg/ml
Sensitivity :
<2pg/ml
Intra-Assay Precision :
Sample 1: n=16; Mean(pg/ml): 83.4; Standard deviation: 3.5; CV(%): 4.2 Sample 2: n=16; Mean(pg/ml): 205; Standard deviation: 12.5; CV(%): 6.1 Sample 3: n=16; Mean(pg/ml): 468; Standard deviation: 19.2; CV(%): 4.1
Inter-Assay Precision :
Sample 1: n=24; Mean(pg/ml): 84.5; Standard deviation: 8.2; CV(%): 9.7 Sample 2: n=24; Mean(pg/ml): 202; Standard deviation: 19.8; CV(%): 9.8 Sample 3: n=24; Mean(pg/ml): 485; Standard deviation: 44.1; CV(%): 9.1
Cross Reactivity :
There is no detectable cross-reactivity with other relevant proteins.
Storage :
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Sample Type :
This kit is used for quantitative detection of human Eotaxin in cell culture supernates, serum and plasma(citrate, EDTA).
Reactivity :
Human
Kit Components :
Catalog number Description Quantity - 96-well plate precoated with anti- human Eotaxin antibody 1 ST0000-10 Lyophilized recombinant human Eotaxin standard 10ng/tubex2 AR1107 Biotinylated anti- human Eotaxin antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml
Background :
Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3(CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin. Recombinant human eotaxin produced in insect cells induces a calcium flux response in normal human eosinophils, but not in neutrophils or monocytes. The human eotaxin gene is cloned and found to be 61.8 and 63.2% identical at the amino acid level to guinea pig and mouse eotaxin.
Gene Name :
CCL11
Protein Name :
Eotaxin
Gene Full Name :
chemokine (C-C motif) ligand 11
Synonyms :
C-C motif chemokine 11 CCL 11 CCL11 CCL11_HUMAN Chemokine (C-C motif) ligand 11 Chemokine C C motif ligand 11 Chemokine CC Motif Ligand 11 Chemokine ligand 11 Eosinophil Chemotactic P Eosinophil chemotactic protein Eotaxin Eotaxin 1 MGC22554 SCYA 11 SCYA11 Small inducible cytokine A11 Small inducible cytokine subfamily A (Cys Cys) member 11 Small inducible cytokine subfamily A member 11 Small-inducible cytokine A11
Uniprot IDs :
P51671
company information
Boster Immunoleader
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
www.bosterbio.com
925.485.4527
headquarters: USA
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