Boster Immunoleader

ELISA/assay

Human EGFR PicoKine ELISA Kit

EK0327

1 x96 well plate

320 USD

Human EGFR PicoKine ELISA Kit

EK0327

320 USD

Sandwich ELISA kit for Quantitative Detection of Human EGFR

NSO, M1-S645

1 x96 well plate

62.5pg/ml-4000pg/ml

<1pg/ml

Sample 1: n=16; Mean(ng/ml): 1.37; Standard deviation: 0.053; CV(%): 3.9 Sample 2: n=16; Mean(ng/ml): 4.68; Standard deviation: 0.211; CV(%): 4.5 Sample 3: n=16; Mean(ng/ml): 6.21; Standard deviation: 0.329; CV(%): 5.3

Sample 1: n=24; Mean(ng/ml): 1.49; Standard deviation: 0.073; CV(%): 4.7 Sample 2: n=24; Mean(ng/ml): 4.92; Standard deviation: 0.32; CV(%): 6.5 Sample 3: n=24; Mean(ng/ml): 7.14; Standard deviation: 0.614; CV(%): 8.6

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human EGFR in cell culture supernates, serum, plasma(heparin, EDTA) and human milk.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human EGFR antibody 1 ST0000-10 Lyophilized recombinant human EGFR standard 10ng/tubex2 AR1107 Biotinylated anti- human EGFR antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

The epidermal growth factor receptor(EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family(EGF-family) of extracellular protein ligands.1 It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR(ErbB-1), HER2/c-neu(ErbB-2), Her 3(ErbB-3) and Her 4(ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha(TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression(known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.

EGFR

Epidermal growth factor receptor

epidermal growth factor receptor

9030024J15Rik AI552599 Avian erythroblastic leukemia viral (v erb b) oncogene homolog Avian erythroblastic leukemia viral (verbb) oncogene homolog Cell growth inhibiting protein 40 Cell proliferation inducing protein 61 EGF R EGFR EGFR_HUMAN Epidermal growth factor receptor Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian)Erbb erbb 1 Erbb1 Erythroblastic leukemia viral (v erb-b) oncogene homolog HER1 mENA Oncogene ERBB OTTMUSP00000005385 OTTMUSP00000005386 PIG61 Proto oncogene c ErbB 1 Proto-oncogene c-ErbB-1 Receptor tyrosine protein kinase ErbB 1 Receptor tyrosine protein kinase ErbB1 Receptor tyrosine-protein kinase ErbB-1 RP23-295E4.1 Urogastrone wa2 Wa5

P00533