product summary
request information :
company name :
Boster Immunoleader
product type :
ELISA/assay
product name :
Mouse EGF PicoKine ELISA Kit
catalog :
EK0326
quantity :
1 x96 well plate
price :
380 USD
product information
Product Name :
Mouse EGF PicoKine ELISA Kit
Catalog Number :
EK0326
Price :
380 USD
Description short :
Sandwich ELISA kit for Quantitative Detection of Mouse EGF
immunogen :
E.coli, N977-R1029
Pack Size :
1 x96 well plate
Assay Range :
15.6pg/ml-1000pg/ml
Sensitivity :
<1pg/ml
Intra-Assay Precision :
Sample 1: n=16; Mean(pg/ml): 82.5; Standard deviation: 5.94; CV(%): 7.2 Sample 2: n=16; Mean(pg/ml): 204; Standard deviation: 11.02; CV(%): 5.4 Sample 3: n=16; Mean(pg/ml): 597; Standard deviation: 32.84; CV(%): 5.5
Inter-Assay Precision :
Sample 1: n=24; Mean(pg/ml): 95.2; Standard deviation: 8.28; CV(%): 8.7 Sample 2: n=24; Mean(pg/ml): 212; Standard deviation: 13.78; CV(%): 6.5 Sample 3: n=24; Mean(pg/ml): 587; Standard deviation: 40.50; CV(%): 6.9
Cross Reactivity :
There is no detectable cross-reactivity with other relevant proteins.
Storage :
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Sample Type :
This kit is used for quantitative detection of mouse EGF in cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates and urine.
Reactivity :
Mouse
Kit Components :
Catalog number Description Quantity - 96-well plate precoated with anti- mouse EGF antibody 1 ST0000-10 Lyophilized recombinant mouse EGF standard 10ng/tubex2 AR1107 Biotinylated anti- mouse EGF antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml
Background :
Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF acts by binding with high affinity to epidermal growth factor receptor(EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.
Gene Name :
EGF
Protein Name :
Pro-epidermal growth factor
Gene Full Name :
epidermal growth factor
Synonyms :
AI790464 Beta urogastrone Epidermal growth factor HOMG4 OTTHUMP00000219721 OTTHUMP00000219722 Pro epidermal growth factor Pro epidermal growth factor precursor (EGF) URG Urogastrone
Uniprot IDs :
P01132
company information
Boster Immunoleader
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
www.bosterbio.com
925.485.4527
headquarters: USA
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