Boster Immunoleader

ELISA/assay

Human EGF PicoKine ELISA Kit

EK0325

1 x96 well plate

320 USD

Human EGF PicoKine ELISA Kit

EK0325

320 USD

Sandwich ELISA kit for Quantitative Detection of Human EGF

E.coli, N971-R1023

1 x96 well plate

4.7pg/ml-300pg/ml

<1pg/ml

Sample 1: n=16; Mean(pg/ml): 42.1; Standard deviation: 1.98; CV(%): 4.7 Sample 2: n=16; Mean(pg/ml): 105; Standard deviation: 5.67; CV(%): 5.4 Sample 3: n=16; Mean(pg/ml): 197; Standard deviation: 6.30; CV(%): 3.2

Sample 1: n=24; Mean(pg/ml): 45.2; Standard deviation: 3.21; CV(%): 7.1 Sample 2: n=24; Mean(pg/ml): 112; Standard deviation: 5.71; CV(%): 5.1 Sample 3: n=24; Mean(pg/ml): 227; Standard deviation: 13.17; CV(%): 5.8

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human EGF in cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human EGF antibody 1 ST0000-10 Lyophilized recombinant human EGF standard 1ng/tubex2 AR1107 Biotinylated anti- human EGF antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF acts by binding with high affinity to epidermal growth factor receptor(EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.

EGF

Pro-epidermal growth factor

epidermal growth factor

AI790464 Beta urogastrone Epidermal growth factor HOMG4 OTTHUMP00000219721 OTTHUMP00000219722 Pro epidermal growth factor Pro epidermal growth factor precursor (EGF) URG Urogastrone

E7EVD2