Boster Immunoleader

ELISA/assay

Rat CNTF PicoKine ELISA Kit

EK0324

1 x96 well plate

333 USD

Rat CNTF PicoKine ELISA Kit

EK0324

333 USD

Sandwich ELISA kit for Quantitative Detection of Rat CNTF

E.coli, A2-M200

1 x96 well plate

31.2pg/ml-2000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 107; Standard deviation: 4.8; CV(%): 4.5 Sample 2: n=16; Mean(pg/ml): 452; Standard deviation: 18.9; CV(%): 4.2 Sample 3: n=16; Mean(pg/ml): 1287; Standard deviation: 50.2; CV(%): 3.9

Sample 1: n=24; Mean(pg/ml): 193; Standard deviation: 16.2; CV(%): 8.4 Sample 2: n=24; Mean(pg/ml): 643; Standard deviation: 52.7; CV(%): 8.2 Sample 3: n=24; Mean(pg/ml): 1057; Standard deviation: 77.2; CV(%): 7.3

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for detection of rat CNTF in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat CNTF antibody 1 ST0000-10 Lyophilized recombinant rat CNTF standard 10ng/tubex2 AR1107 Biotinylated anti- rat CNTF antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Ciliary neurotrophic factor(CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The hCNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid(aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85% homology with the deduced aa sequence encoding hCNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.

CNTF

Ciliary neurotrophic factor

ciliary neurotrophic factor

AI429687 Ciliary Neurotrophic Factor HCNTF MGC41235 OTTHUMP00000174731 OTTMUSP00000029848

P20294