Boster Immunoleader

ELISA/assay

Human BMP-4 PicoKine ELISA Kit

EK0314

1 x96 well plate

400 USD

Human BMP-4 PicoKine ELISA Kit

EK0314

400 USD

Sandwich ELISA kit for Quantitative Detection of Human BMP-4

NSO, S293-R408

1 x96 well plate

62.5pg/ml-4000pg/ml

<4pg/ml

Sample 1: n=16; Mean(pg/ml): 376; Standard deviation: 15.79; CV(%): 4.2 Sample 2: n=16; Mean(pg/ml): 987; Standard deviation: 45.40; CV(%): 4.6 Sample 3: n=16; Mean(pg/ml): 2136; Standard deviation: 117.48; CV(%): 5.5

Sample 1: n=24; Mean(pg/ml): 453; Standard deviation: 23.10; CV(%): 5.1 Sample 2: n=24; Mean(pg/ml): 1209; Standard deviation: 68.91; CV(%): 5.7 Sample 3: n=24; Mean(pg/ml): 2351; Standard deviation: 141.06; CV(%): 6.0

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human BMP-4 in bone tissue and cell culture supernates.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human BMP-4 antibody 1 ST0000-10 Lyophilized recombinant human BMP-4 standard 10ng/tubex2 AR1107 Biotinylated anti- human BMP-4 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23.1, The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog(released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal(by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. The standard product used in this kit is recombinant BMP-4 with the molecular mass of 26KDa.

BMP4

Bone morphogenetic protein 4

bone morphogenetic protein 4

BMP 2B BMP 4 BMP2B BMP2B1 BOMPR4A Bone morphogenetic protein 2B Bone morphogenetic protein 4 DVR4 OTTMUSP00000021065 OTTMUSP00000021066 ZYME

P12644