Boster Immunoleader

ELISA/assay

Human BDNF PicoKine ELISA Kit

EK0307

1 x96 well plate

270 USD

Human BDNF PicoKine ELISA Kit

EK0307

270 USD

Sandwich ELISA kit for Quantitative Detection of Human BDNF

sf21, H129-R247

1 x96 well plate

31.2pg/ml-2000pg/ml

<2pg/ml

Sample 1: n=16; Mean(pg/ml): 113; Standard deviation: 3.96; CV(%): 3.5 Sample 2: n=16; Mean(pg/ml): 425; Standard deviation: 17.85; CV(%): 4.2 Sample 3: n=16; Mean(pg/ml): 1278; Standard deviation: 62.62; CV(%): 4.9

Sample 1: n=24; Mean(pg/ml): 197; Standard deviation: 14.18; CV(%): 7.2 Sample 2: n=24; Mean(pg/ml): 594; Standard deviation: 44.55; CV(%): 7.5 Sample 3: n=24; Mean(pg/ml): 1306; Standard deviation: 103.17; CV(%): 7.9

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human BDNF in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human BDNF antibody 1 ST0000-10 Lyophilized recombinant human BDNF standard 10ng/tubex2 AR1107 Biotinylated anti- human BDNF antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.

BDNF

Brain-derived neurotrophic factor

brain-derived neurotrophic factor

Abrineurin ANON2 Brain derived neurotrophic factor BULN2 MGC34632 Neurotrophin

P23560