Boster Immunoleader

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Dual Color protein Loading Buffer (4x)

AR1142

6 ml

15 USD

AR1142

Dual Color protein Loading Buffer (4x)

6 ml

15 USD

Western Blotting Related Reagent

4˚C. And for longer storage, please store at -20˚C.

Dual Color Protein Loading Buffer is a complete buffer solution system. This optimized composition of the loading buffer prevents protein degradation during sample heating prior to SDS-PAGE and electrophoresis, as well against pH changes during the SDS-PAGE run (Many proteins are sensitive to pH changes that result from temperature fluctuations of Tris buffers during electrophoresis). It contains two tracking dyes: blue (bromophenol blue) for tracking the progress of electrophoresis and pink (pyronin Y) for monitoring of protein transfer to the membrane during Western blotting procedures. Dual Color Protein Loading Buffer also contains SDS and DTT for complete disruption of all high-order protein structures.

1. Mix sample with Dual Color Protein Loading Buffer 4X at the ratio 3:1 (i.e. add 3g sample to 1ml Dual Color Protein Loading Buffer). Denaturize at 100˚C for 3-5 min. Then load and electrophorese. 2. Stop electrophoresis when dye arrives at the bottom of gels. Note 1. If SDS sediments appeared when balance Dual Color Protein Loading Buffer 4X to room temperature, try to melt it with warm water. 2. Please operate with gloves in fume hood.

For protein sample processing before SDS－PAGE electrophoresis

18ml protein solution