Boster Immunoleader

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WB Strippping Buffer

AR0153

100 ml

60 USD

AR0153

WB Strippping Buffer

100 ml

60 USD

Western Blotting Related Reagent

Store at room temperature for frequent use. Avoid light and multiple freeze-thaw cycles.

Boster's WB Stripping Buffer is used of the recycle of deproteinized membrane in WB. After the conjugation of the Primary and Secondary Antibodies as well as later chemiluminescence, it is recommoned to detect other proteins with stable expression level like Actin and Tubulin etc as a reference. The special component in WB Stripping Buffer will dissolve the conjugation between Primary, Secondary Antibodies and antigens. Comparing wtih the SDS-PAGE gel, it is time-saving and accurate.

1. Wash membrane with TBS-T (3 times, 10 min each). Immerse into WB Stripping Buffer. Incubate at room temperature for 15-30 min and shake slowly.(Note:lengthen washing time of some antibodies). 2. Take out membrane with tweezer. Elute with TBS-T and wash for 5 min. 3. Seal with defatted milk powder or BSA, prepare for next antibodies' incubation.

For the recycle of deproteinized membrane in WB

10ml per membrane, about 10 times