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Imprinted Membrane Fast Reversible Protein Staining Reagent

AR0142

12 ml

10 USD

AR0142

Imprinted Membrane Fast Reversible Protein Staining Reagent

12 ml

10 USD

Western Blotting Related Reagent

At room temperature for one year.

Imprinted membrane fast reversible protein staining reagent can be used for protein detection on PVDF and nitrocellulose membrane. Since the dye of this reagent is with negative charge, it can combine with amino-acid residue with positive charge. Meanwhile, it also can combine with non-polar area of protein to form red bands thereby. For the reversible protein staining, protein can be cleaned again by washing with distilled water, PBS buffer or other appropriate solution. With advantages of convenient use, low background and high sensitivity, this product can detect protein at minimum 250ng, but it is not suitable for protein detection on nylon membrane.

1. Dilute this reagent at 1:10 to make it as working reagent. 2. Immerse the PVDF or nitrocellulose membrane in the above working reagent. Shake for 3-5 min or longer time until bright bands showed, and record the results. 3. Rinse with distilled water, PBS buffer or other appropriate solution for 2-3 times, 3-5 minutes each to remove the dye, and continue the following western blotting steps.

For protein detection on PVDF membrane and nitrocellulose membrane

10 NC membrane（5*8.5cm）