Boster Immunoleader

other

SDS-PAGE Gel Preparation Kit

AR0138

1 kit

45 USD

AR0138

SDS-PAGE Gel Preparation Kit

1 kit

45 USD

Western Blotting Related Reagent

Store 1.5 M Tris-HCl, pH8.8, 10% SDS, Ammonnium persulfate and 1M Tris-HCl, pH 6.8 at room temperature. Store 30% Acr-Bis (29:1) and TEMED at 4˚C in dark. Dilute Ammonnium persulfate with DDH2O (double distilled water) at 1:10. Then aliquot it into small tubes, and store at -20˚C(can be valid for about a half of one year).

Boster’s SDS-PAGE Gel Preparation Kit is convenient to use. It contains various reagents for SDS-PAGE Gel Preparation, only the experimental instruments and DDH2O are needed to prepare. It not only can make up SDS-PAGE gel, but also non-native PAGE gel. This Kit is enough for 30-50 pieces of normal size PAGE gel.

1. Choose the proper concentration of gel according to the molecular weight of target protein. Working range: Molecular weight of target protein (Da) Proper gel concentration (%) <104 20-30 1x104-4x104 15-20 4x104-1x105 10-15 1x105-5x105 5-10 >5x105 2-5 2 . SDS-PAGE separation gel Electrophoresis running gel concentration Reagent 10% 12% 15% 10% 12% 15% DDH2O(ml) 4.0 3.3 2.3 5.9 4.9 3.4 30%Acr-Bis (T: 30%,C: 3%)(ml) 3.3 4.0 5.0 5.0 6.0 7.5 1.5mol/lTris.cl(pH8.8) (ml) 2.5 2.5 2.5 3.8 3.8 3.8 10%SDS(ml) 0.1 0.1 0.1 0.15 0.15 0.15 10% Ammonium persulfate (ml) 0.1 0.1 0.1 0.15 0.15 0.15 TEMED(ul) 4 4 4 6 6 6 Total volume(ml) 10 15 3 . SDS-PAGE spacer gel (Concentration: 5%) Reagent Scheme 1 Scheme 2 DDH2O(ml) 4 2 30% Acr-Bis (T: 30%,C: 3%(ml) 1 0.5 1.0mol/lTris.cl(pH6.8) (ml) 1 0.5 10%SDS(ul) 80 40 10% Ammonium persulfate (ul) 60 30 TEMED(ul) 8 4 Total volume (ml) 6 3

For making up SDS-PAGE gel, as well as native PAGE gel

Enough for 30-50 pieces of gel