Boster Immunoleader

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Mammal Cell Protein Extraction Reagent

AR0103

100 ml

70 USD

AR0103

Mammal Cell Protein Extraction Reagent

100 ml

70 USD

Western Blotting Related Reagent

Store at 4˚C for frequent use, at -20˚C for at least one year.

This reagent is bland. To avoid protein degradation by human factor, the preparation should be operated in low temperature and add proper protease inhibitor. (Use 1ml for each time. Temporary use should add 100mm PMSF 5.7ul which has high neurotoxicity and absorb easily to skin.)

1. For anchorage-dependent cell Remove the cultural solution, wash with PBS, normal saline or cultural solution without serum. Add proper lysate and operate with peptide to make the cell and lysate contact fully. Usually, the cell will be cleaved after 10 seconds. 2. For suspended cell Contrifuge to collect cells. Wash with PBS, normal saline or cultural solution without serum. Add proper lysate and operate with peptide to blow off the cell. Percuss with finger to make the cell cleaved fully. It’s not supposed to see clear cell precipitate after full cleavage. If too many cells received, subpackage and cleavage. 3. After full cleavage 10000-14000 g contrifuge for 3-5 minutes. Get the supernatant solution, and do the following operation.

For protein extraction in WB

centrifuge and precipitate about 20ml cells