Mammal Tissue Protein Extraction Reagent | Boster Immunoleader AR0101 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

other

product name :

Mammal Tissue Protein Extraction Reagent

catalog :

AR0101

quantity :

100 ml

price :

100 USD

publications citing this reagent :

Gui J, Xiong F, Li J, Huang G. Effects of acupuncture on Th1, th2 cytokines in rats of implantation failure. Evid Based Complement Alternat Med. 2012;2012:893023 pubmed

more info or order :

Boster Immunoleader product webpage

product information

Code :

AR0101

Name :

Mammal Tissue Protein Extraction Reagent

Size :

100 ml

Price :

100 USD

SubCategory :

Western Blotting Related Reagent

Storage :

Store at 4˚C for frequent use, at -20˚C for at least one year.

Indtroduction :

This reagent is bland. Add a small amount of detergent when cytolyse, and the detergent can be dialyzed. Additionally, this reagent has no effect on the detection of standard protein.

Protocol :

1. Place the fresh tissue into pre-cooling normal saline and wash several times to clean the blood on the surface. Weigh the tissue samples, cut into pieces before adding to a glass homogenizer. 2. Homogenize tissue samples in 1ml of extraction reagent per 0.1g of tissue (Increase the amount of extraction reagent if the samples were not lysed completely; Reduce the amount of extraction reagent if need protein samples with high concentration). Add PMSF (100mmol/ml) before using and dilute to 1m mol/ml. 3. Homogenization using a glass homogenizer until the tissue samples were lysed completely. 4. Centrifuge for 3-5 minutes at 10000-14000 g. Get the supernatant solution, and do the next steps.

Application :

For protein extraction in WB

Number of assays :

lyse about 10g tissue

more info or order :

Boster Immunoleader product webpage