protein

Recombinant Human B3GAT1 (N-6His)

C11D

10 ug;50 ug;500 ug;1 mg

101 USD

Recombinant Human B3GAT1 (N-6His)

protein

Q9P2W7

10 ug;50 ug;500 ug;1 mg

101 USD

C11D-10ug

10ug;50ug;500ug;1mg

Recombinant Human Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 is produced by our Mammalian expression system and the target gene encoding His25-Ile334 is expressed with a 6His tag at the N-terminus.

Human Cells

B3GAT1 is the key enzyme during the biosynthesis of the carbohydrate epitope HNK-1, which is present on a number of cell adhesion molecules important in neurodevelopment. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta 14GlcNAc) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK1 epitope, a unique trisaccharide structure, HSO3-3GlcA beta 1-3Gal beta 1-4GlcNAc. The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol. The HNK1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell-cell and cell-substratum interaction and recognition during the development of the nervous system. Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N-terminal cytoplasmic domain and a single pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method.

Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

Lyophilized from a 0.2 μm filtered solution of 20mM Tris HCl, 150mM NaCl, pH8.0.

Ambient

B3GAT1; beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P); CD57; GlcAT-P; HNK1; NK1; NK-1