AKT1+2+3 (Tyr315+316+312) Antibody | Bioss bs-5193R product information

product summary

company name :

Bioss

product type :

antibody

product name :

AKT1+2+3 (Tyr315+316+312) Antibody

catalog :

bs-5193R

quantity :

100 ul

price :

309.5 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Bioss product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 3e	Wang X, Shan Y, Tan Q, Tan C, Zhang H, Liu J, et al. MEX3A knockdown inhibits the development of pancreatic ductal adenocarcinoma. Cancer Cell Int. 2020;20:63 pubmed publisher
flow cytometry; human	Iriyama N, Hatta Y, Takei M. Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes. Cancer Med. 2016;5:3223-3234 pubmed publisher

product information

sku :

bs-5193R

name :

AKT1+2+3 (Tyr315+316+312) Antibody

category :

Primary Antibody

conjugation :

Unconjugated

host :

Rabbit

target protein :

AKT1+2+3 Tyr315+316+312

specificity :

This antibody will detect AKT 1 when phosphorylated at Tyr315 in Human, Mouse, Rat or Bovine; AKT2 when phosphorylated at Tyr316 in Human, Mouse, Rat, or Bovine; and AKT3 when phosphorylated at Tyr312 in Human, Mouse, Rat, or Bovine.

modification :

Phosphorylation

modification site :

Tyr315+316+312

clonality :

Polyclonal

isotype :

IgG

concentration :

1ug/ul

public immunogen range :

280-330/480

subcellular locations :

Cytoplasm, Nucleus, Cell membrane

source :

KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Tyr315

gene id link :

Entrez Gene: 207, 208, 10000

applications :

WB, IHC-P, IF(IHC-P)

applications with dilutions :

WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)

cross reactive species :

Human, Mouse, Rat

size :

100 ul

price :

309.5 USD

background :

AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.

purification :

Purified by Protein A.

storage :

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

synonyms :

AKT1/AKT2/AKT3 Tyr315+Tyr316+Tyr312; AKT1/AKT2/AKT3Tyr315/316/312; AKT1/2/3 phospho Y315 + Y316 + Y312; AKT 1; AKT; AKT1; AKT-1; AKT1_HUMAN; AKT 2; AKT2; AKT-2; AKT2_HUMAN; AKT 3; AKT3; AKT-3; AKT3_HUMAN; C AKT; cAKT; MGC9965; MGC99656; Oncogene AKT1; PKB; PKB alpha; PKB-ALPHA; PKB beta; PKB gamma; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; RAC Alpha; RAC alpha serine/threonine protein kinase; RAC; RAC PK Alpha; Rac protein kinase alpha; RAC Serine/Threonine Protein Kinase; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; v akt murine thymoma viral oncogene homolog 1; vAKT Murine Thymoma Viral Oncogene Homolog 1.

lead time :

3 to 5 business days.

more info or order :

Bioss product webpage