Smad3 (Ser423 + Ser425) Antibody | Bioss bs-3425R product information

product summary

company name :

Bioss

product type :

antibody

product name :

Smad3 (Ser423 + Ser425) Antibody

catalog :

bs-3425R

quantity :

100 ul

price :

309.5 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, pig

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Bioss product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; pig	Bai L, Chu G, Wang W, Xiang A, Yang G. BAMBI promotes porcine granulosa cell steroidogenesis involving TGF-β signaling. Theriogenology. 2017;100:24-31 pubmed publisher
western blot; rat	Shang P, Liu W, Liu T, Zhang Y, Mu F, Zhu Z, et al. Acetyl-11-Keto-β-Boswellic Acid Attenuates Prooxidant and Profibrotic Mechanisms Involving Transforming Growth Factor-β1, and Improves Vascular Remodeling in Spontaneously Hypertensive Rats. Sci Rep. 2016;6:39809 pubmed publisher
western blot; human; 1:500; fig 5	Ghiabi P, Jiang J, Pasquier J, Maleki M, Abu-Kaoud N, Halabi N, et al. Breast cancer cells promote a notch-dependent mesenchymal phenotype in endothelial cells participating to a pro-tumoral niche. J Transl Med. 2015;13:27 pubmed publisher

product information

sku :

bs-3425R

name :

Smad3 (Ser423 + Ser425) Antibody

category :

Primary Antibody

conjugation :

Unconjugated

host :

Rabbit

target protein :

Smad3 Ser423 + Ser425

specificity :

These phosphorylation sites are homologous across the species listed.

modification :

Phosphorylation

modification site :

Ser423 + Ser425

clonality :

Polyclonal

isotype :

IgG

concentration :

1ug/ul

public immunogen range :

380-425/425

subcellular locations :

Cytoplasm, Nucleus

source :

KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425 [CS(p-S)V(p-S)]

gene id link :

Entrez Gene: 4088

swiss prot :

P84022

swiss prot link :

Swiss Prot: P84022

applications :

WB, IHC-P, IF(IHC-P)

applications with dilutions :

WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)

cross reactive species :

Human, Mouse, Rat

size :

100 ul

price :

309.5 USD

background :

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

purification :

Purified by Protein A.

storage :

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

synonyms :

LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD family member 3; SMAD 3; Smad3; hSMAD3

lead time :

3 to 5 business days.

more info or order :

Bioss product webpage