PKR (Thr446 + Thr451) Antibody | Bioss bs-3337R product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Bioss

product type :

antibody

product name :

PKR (Thr446 + Thr451) Antibody

catalog :

bs-3337R

quantity :

100 ul

price :

299 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

product information

sku :

bs-3337R

name :

PKR (Thr446 + Thr451) Antibody

category :

Primary Antibody

conjugation :

Unconjugated

host :

Rabbit

target protein :

PKR Thr446 + Thr451

modification :

Phosphorylation

modification site :

Thr446 + Thr451

clonality :

Polyclonal

isotype :

IgG

concentration :

1ug/ul

public immunogen range :

420-470/551

subcellular locations :

Cytoplasm, Nucleus

source :

KLH conjugated synthetic phosphopeptide derived from human PKR around the phosphorylation site of Thr446/451

gene id link :

Entrez Gene: 5610

swiss prot :

P19525

swiss prot link :

Swiss Prot: P19525

applications :

WB, IHC-P, IF(IHC-P)

applications with dilutions :

WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)

cross reactive species :

Human

size :

100 ul

price :

299 USD

background :

IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis. Also phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2).

purification :

Purified by Protein A.

storage :

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

synonyms :

PKR; PRKR; EIF2AK1; PPP1R83; Interferon-induced, double-stranded RNA-activated protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 2; eIF-2A protein kinase 2; Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein kinase; Protein kinase RNA-activated; Protein kinase R; Tyrosine-protein kinase EIF2AK2; p68 kinase; EIF2AK2

lead time :

3 to 5 business days.