IKK alpha / beta (S176 / S180) Antibody | Bioss bs-3237R product information

product summary

company name :

Bioss

product type :

antibody

product name :

IKK alpha / beta (S176 / S180) Antibody

catalog :

bs-3237R

quantity :

100 ul

price :

309.5 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Bioss product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; mouse	Gao W, Guo Y, Wang C, Lin Y, Yu L, Sheng T, et al. Indirubin ameliorates dextran sulfate sodium-induced ulcerative colitis in mice through the inhibition of inflammation and the induction of Foxp3-expressing regulatory T cells. Acta Histochem. 2016;118:606-14 pubmed publisher
western blot; rat	Zhao H, Zheng Q, Hu X, Shen H, Li F. Betulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-κB signaling pathway. Life Sci. 2016;144:185-93 pubmed publisher

product information

sku :

bs-3237R

name :

IKK alpha / beta (S176 / S180) Antibody

category :

Primary Antibody

conjugation :

Unconjugated

host :

Rabbit

target protein :

IKK alpha/beta Ser176 / Ser180

specificity :

These phosphorylation sites are homologous across the listed species.

modification :

Phosphorylation

modification site :

Ser176 + Ser180

clonality :

Polyclonal

isotype :

IgG

concentration :

1ug/ul

public immunogen range :

150-200/756

subcellular locations :

Nucleus

source :

KLH conjugated synthetic phosphopeptide derived from human IKK alpha/beta around the phosphorylation site of Ser176/Ser180

gene id link :

Entrez Gene: 1147

swiss prot :

O15111

swiss prot link :

Swiss Prot: O15111

applications :

WB, IHC-P, IF(IHC-P)

applications with dilutions :

WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)

cross reactive species :

Human, Mouse, Rat

size :

100 ul

price :

309.5 USD

background :

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3.

purification :

Purified by Protein A.

storage :

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

synonyms :

IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; Nuclear factor NF-kappa-B inhibitor kinase alpha; Transcription factor 16; TCF-16; CHUK

lead time :

3 to 5 business days.

more info or order :

Bioss product webpage