EGFRv3 Polyclonal Antibody | Bioss bs-2558R product information

product summary

company name :

Bioss

product type :

antibody

product name :

EGFRv3 Polyclonal Antibody

catalog :

bs-2558R

quantity :

100 ul

price :

289.5 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Bioss product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human	Wang F, Kang C, Wang-Gou S, Huang C, Feng C, Li X. EGFL7 is an intercellular EGFR signal messenger that plays an oncogenic role in glioma. Cancer Lett. 2017;384:9-18 pubmed publisher
immunohistochemistry - paraffin section; human; loading ...; fig 1	Khelwatty S, Essapen S, Bagwan I, Green M, Seddon A, Modjtahedi H. The impact of co-expression of wild-type EGFR and its ligands determined by immunohistochemistry for response to treatment with cetuximab in patients with metastatic colorectal cancer. Oncotarget. 2016;: pubmed publisher
immunocytochemistry; mouse western blot; mouse	Wei J, Cui J, Zhou X, Fang C, Tan Y, Chen L, et al. F25P preproinsulin abrogates the secretion of pro-growth factors from EGFRvIII cells and suppresses tumor growth in an EGFRvIII/wt heterogenic model. Cancer Lett. 2016;380:1-9 pubmed publisher
immunohistochemistry - frozen section; mouse	Wehrenberg-Klee E, Redjal N, Leece A, Turker N, Heidari P, Shah K, et al. PET imaging of glioblastoma multiforme EGFR expression for therapeutic decision guidance. Am J Nucl Med Mol Imaging. 2015;5:379-89 pubmed
western blot; human	Agnes R, Broome A, Wang J, Verma A, Lavik K, Basilion J. An optical probe for noninvasive molecular imaging of orthotopic brain tumors overexpressing epidermal growth factor receptor. Mol Cancer Ther. 2012;11:2202-11 pubmed publisher

product information

sku :

bs-2558R

name :

EGFRv3 Polyclonal Antibody

category :

Primary Antibody

conjugation :

Unconjugated

host :

Rabbit

target protein :

EGFRv3

modification :

Unmodified

clonality :

Polyclonal

isotype :

IgG

concentration :

1ug/ul

public immunogen range :

1-50/1081

subcellular locations :

Extracellular

source :

KLH conjugated synthetic peptide derived from human EGFRvIII

gene id link :

Entrez Gene: 1956

swiss prot :

P00533

swiss prot link :

Swiss Prot: P00533

applications :

WB, IHC-P, IF(IHC-P)

applications with dilutions :

WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)

cross reactive species :

Human

size :

100 ul

price :

289.5 USD

background :

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.

purification :

Purified by Protein A.

storage :

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

synonyms :

ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR

lead time :

3 to 5 business days.

more info or order :

Bioss product webpage