PAK4/5(Ser99) Polyclonal Antibody | Bioss bs-2270R product information

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product summary

company name :

Bioss

product type :

antibody

product name :

PAK4/5(Ser99) Polyclonal Antibody

catalog :

bs-2270R

quantity :

100 ul

price :

299 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

product information

sku :

bs-2270R

name :

PAK4/5(Ser99) Polyclonal Antibody

category :

Primary Antibody

conjugation :

Unconjugated

host :

Rabbit

target protein :

PAK4/5 Ser99

specificity :

This phosphorylation site is homologous in the listed cross reactive species at the specified location.

modification :

Phosphorylation

modification site :

Ser99

clonality :

Polyclonal

isotype :

IgG

concentration :

1ug/ul

public immunogen range :

80-120/591

subcellular locations :

Cytoplasm, Nucleus, Mitochondrion

source :

KLH conjugated synthetic phosphopeptide derived from human PAK4 around the phosphorylation site of Ser99

gene id link :

Entrez Gene: 10298, 57144

applications :

WB, IHC-P, IF(IHC-P)

applications with dilutions :

WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)

cross reactive species :

Human, Mouse, Rat

size :

100 ul

price :

299 USD

background :

p21-activated kinases (PAKs) belong to the family of serine/threonine kinases involved in the control of various cellular processes, including the cell cycle, dynamics of the cytoskeleton, apoptosis, oncogenic transformation, and transcription. All PAK family members are characterized by the presence of p21-binding domain. p21-activated kinases are regulated by the small GTP-binding proteins Rac and Cdc42, and lipids, which stimulate autophosphorylation and phosphorylation of exogenous substrates. Serine (Ser-474) is the likely autophosphorylation site in the kinase domain of PAK4 in vivo. Phosphospecific directed against serine 474 detect activated PAK4 on the Golgi membrane when PAK4 is co-expressed with activated Cdc42. Current data strongly implicates PAK-4 in oncogenesis. PAK4 is frequently overexpressed in human tumor cell lines of various tissue origins. Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions.

purification :

Purified by Protein A.

storage :

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

synonyms :

p21 activated kinase 4 ; p21CDKN1A activated kinase 4; PAK 4; Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs; Serine threonine kinase PAK 4; Serine/threonine protein kinase PAK 4; Serine/threonine protein kinase PAK4; KIAA1142; Serine/threonine-protein kinase PAK 7; PAK7;

lead time :

3 to 5 business days.