Brk (Tyr447) Polyclonal Antibody | Bioss bs-12890R product information

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product summary

company name :

Bioss

product type :

antibody

product name :

Brk (Tyr447) Polyclonal Antibody

catalog :

bs-12890R

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

product information

sku :

bs-12890R

name :

Brk (Tyr447) Polyclonal Antibody

category :

Primary Antibody

conjugation :

Unconjugated

host :

Rabbit

target protein :

Brk Tyr447

modification :

Phosphorylation

modification site :

Tyr447

clonality :

Polyclonal

isotype :

IgG

concentration :

1ug/ul

public immunogen range :

438-451/451

subcellular locations :

Cytoplasm, Nucleus

source :

KLH conjugated synthetic phosphopeptide derived from human Brk around the phosphorylation site of Tyr447 [TS(p-Y)EN]

gene id link :

Entrez Gene: 5753

swiss prot :

Q13882

swiss prot link :

Swiss Prot: Q13882

applications :

WB, IHC-P, IF(IHC-P)

applications with dilutions :

WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)

cross reactive species :

Human

size :

100 ul

background :

Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p19RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p19RhoGAP, which promotes association with RASA1/p12RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways. Isoform 2 inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins.

purification :

Purified by Protein A.

storage :

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

synonyms :

BRK; Protein-tyrosine kinase 6; Breast tumor kinase; Tyrosine-protein kinase BRK; PTK6

lead time :

3 to 5 business days.