NFKB p65(Ser536) Polyclonal Antibody | Bioss bs-0982R product information

product summary

company name :

Bioss

product type :

antibody

product name :

NFKB p65(Ser536) Polyclonal Antibody

catalog :

bs-0982R

quantity :

100 ul

price :

309.5 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Bioss product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; rat	Sun G, Yang W, Zhang Y, Zhao M. Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats. Exp Anim. 2017;66:303-312 pubmed publisher
western blot; mouse	Gao W, Guo Y, Wang C, Lin Y, Yu L, Sheng T, et al. Indirubin ameliorates dextran sulfate sodium-induced ulcerative colitis in mice through the inhibition of inflammation and the induction of Foxp3-expressing regulatory T cells. Acta Histochem. 2016;118:606-614 pubmed publisher
western blot; rat	Zhang L, Zhao S, Yuan L, Wu H, Jiang H, Luo G, et al. Knockdown of placental growth factor (PLGF) mitigates hyperoxia-induced acute lung injury in neonatal rats: Suppressive effects on NF?B signaling pathway. Int Immunopharmacol. 2016;38:167-74 pubmed publisher
western blot; rat	Fu J, Shi Q, Song X, Xia X, Su C, Liu Z, et al. Tetrachlorobenzoquinone exhibits neurotoxicity by inducing inflammatory responses through ROS-mediated IKK/IÎºB/NF-ÎºB signaling. Environ Toxicol Pharmacol. 2016;41:241-50 pubmed publisher
western blot; rat	Zhao H, Zheng Q, Hu X, Shen H, Li F. Betulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-ÎºB signaling pathway. Life Sci. 2016;144:185-93 pubmed publisher
western blot; rat	Zhao H, Liu Z, Liu W, Han X, Zhao M. Betulin attenuates lung and liver injuries in sepsis. Int Immunopharmacol. 2016;30:50-56 pubmed publisher
western blot; mouse	Yan X, Wu L, Li B, Meng X, Dai H, Zheng Y, et al. Cyanidin-3-O-glucoside attenuates acute lung injury in sepsis rats. J Surg Res. 2015;199:592-600 pubmed publisher

product information

sku :

bs-0982R

name :

NFKB p65(Ser536) Polyclonal Antibody

category :

Primary Antibody

conjugation :

Unconjugated

host :

Rabbit

target protein :

NFKB p65 Ser536

modification :

Phosphorylation

modification site :

Ser536

clonality :

Polyclonal

isotype :

IgG

concentration :

1ug/ul

public immunogen range :

515-551/551

subcellular locations :

Cytoplasm, Nucleus

source :

KLH conjugated synthetic phosphopeptide derived from human NFKBp65 around the phosphorylation site of(Ser536)

gene id link :

Entrez Gene: 5970

swiss prot :

Q04206

swiss prot link :

Swiss Prot: Q04206

applications :

WB, IHC-P, IF(IHC-P)

applications with dilutions :

WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)

cross reactive species :

Human, Mouse, Rat

size :

100 ul

price :

309.5 USD

background :

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681).

purification :

Purified by Protein A.

storage :

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

synonyms :

p65; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; RELA

lead time :

3 to 5 business days.

more info or order :

Bioss product webpage