NFKB p65 Polyclonal Antibody | Bioss bs-0465R product information

product summary

company name :

Bioss

product type :

antibody

product name :

NFKB p65 Polyclonal Antibody

catalog :

bs-0465R

quantity :

100 ul

price :

289.5 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

unidentified, human, mouse, rat, domestic rabbit

application :

western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Bioss product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; fig 2g	Vijayaraj P, Minasyan A, Durra A, Karumbayaram S, Mehrabi M, Aros C, et al. Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule. Cell Rep. 2019;29:3488-3505.e9 pubmed publisher
immunohistochemistry - paraffin section; mouse	Fattori V, Borghi S, Guazelli C, Giroldo A, Crespigio J, Bussmann A, et al. Vinpocetine reduces diclofenac-induced acute kidney injury through inhibition of oxidative stress, apoptosis, cytokine production, and NF-?B activation in mice. Pharmacol Res. 2017;120:10-22 pubmed publisher
immunohistochemistry - paraffin section; unidentified	Xi L, Hu R, Guo T, Wang Y, Sheng X, Han Y, et al. Immunoreactivities of NF-?B, IL-1? and IL-1R in the skin of Chinese brown frog (Rana dybowskii). Acta Histochem. 2017;119:64-70 pubmed publisher
immunohistochemistry - paraffin section; rat	Oyagbemi A, Omobowale T, Asenuga E, Adejumobi A, Ajibade T, Ige T, et al. Sodium fluoride induces hypertension and cardiac complications through generation of reactive oxygen species and activation of nuclear factor kappa beta. Environ Toxicol. 2017;32:1089-1101 pubmed publisher
western blot; domestic rabbit; fig 4	Yang Z, Zhong Z, Li M, Xiong Y, Wang Y, Peng G, et al. Hypothermic machine perfusion increases A20 expression which protects renal cells against ischemia/reperfusion injury by suppressing inflammation, apoptosis and necroptosis. Int J Mol Med. 2016;38:161-71 pubmed publisher
western blot; human	Ganguly R, Wen A, Myer A, Czech T, Sahu S, Steinmetz N, et al. Anti-atherogenic effect of trivalent chromium-loaded CPMV nanoparticles in human aortic smooth muscle cells under hyperglycemic conditions in vitro. Nanoscale. 2016;8:6542-54 pubmed publisher
	Chen H, Wang H, Yu X, Zhou S, Zhang Y, Wang Z, et al. ERCC6L promotes the progression of hepatocellular carcinoma through activating PI3K/AKT and NF-κB signaling pathway. BMC Cancer. 2020;20:853 pubmed publisher
	Zheng J, Liu Z, Li W, Tang J, Zhang D, Tang X. Lithium posttreatment confers neuroprotection through glycogen synthase kinase-3? inhibition in intracerebral hemorrhage rats. J Neurosurg. 2017;127:716-724 pubmed publisher

product information

sku :

bs-0465R

name :

NFKB p65 Polyclonal Antibody

category :

Primary Antibody

conjugation :

Unconjugated

host :

Rabbit

target protein :

NFKB p65

modification :

Unmodified

clonality :

Polyclonal

isotype :

IgG

concentration :

1ug/ul

public immunogen range :

50-100/551

subcellular locations :

Cytoplasm

source :

KLH conjugated synthetic peptide derived from human NFKBp65

gene id link :

Entrez Gene: 5970

swiss prot :

Q04206

swiss prot link :

Swiss Prot: Q04206

applications :

WB, FCM, IHC-P, IF(IHC-P)

applications with dilutions :

WB(1:100-1000), FCM(1:20-100), IHC-P(1:100-500), IF(IHC-P)(1:50-200)

cross reactive species :

Human, Mouse, Rat, Pig

size :

100 ul

price :

289.5 USD

background :

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p15, NFKB1/p5, REL and NFKB2/p52 and the heterodimeric p65-p5 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p5 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

purification :

Purified by Protein A.

storage :

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

synonyms :

p65; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; RELA

lead time :

3 to 5 business days.

more info or order :

Bioss product webpage