rabbit polyclonal
reactivity: human, mouse, rat, dog
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 2
reactivity: human, mouse, rat, dog
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 2
Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-PKC alpha/beta II (Thr638/641) Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Formalin-fixed and paraffin embedded mouse brain labeled with Rabbit Anti-PKC alpha/beta II (Thr638/641) Polyclonal Antibody, Unconjugated (bs-3333R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
quantity: 100 ul
price: 309.5 USD
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat, dog
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
citations: 2
reactivity: human, mouse, rat, dog
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
citations: 2
Image Kindly Submitted from Dr. Magdalena Krol. Protein expression was assessed using western blotting. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Nuclear and cytoplasmic protein extracts from cultured cells were isolated by lysis using the CelLytic NuCLEAR reagent (Sigma Aldrich), according to the manufacturer’s instructions. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-PKC alpha/beta/gamma Polyclonal Antibody (bs-3531R) at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000. An Odyssey Infrared Imaging System (LI-COR Biosciences, USA) was then used to analyze protein expression. Scan resolution and intensity of the instrument were set at 169 mm and 4, respectively. Quantification of the integrated optical density (IOD) was performed using the analysis software provided with the Odyssey scanner (LI-COR Biosciences). To remove antibodies, the membranes were incubated for 15 min at room temperature in Restore Western Blot Stripping Buffer (Thermo Scientific, USA). This experiment was repeated seven times.
quantity: 100 ul
price: 279 USD
to the supplier