Sheep antibody to alpha synuclein (116-131): affinity purified

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product summary

company name :

Biosensis

product type :

antibody

product name :

Sheep antibody to alpha synuclein (116-131): affinity purified

catalog :

S-075-50

quantity :

50 µg

clonality :

polyclonal

host :

domestic sheep

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

product information

Catalog Number :

S-075-50

Product Name :

Sheep antibody to alpha synuclein (116-131): affinity purified

Host Species :

Sheep

Clonality :

Polyclonal

Product Type :

Purified

Size :

50 µg

list of Pubmed id :

20106867, 20533994, 18385100

Product Description :

Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. Alpha synuclein has been associated with several neurodegenerative diseases. A point mutation in the gene coding for the alpha-synuclein protein was the first discovery linking this protein to a rare familial form of Parkinson's disease (PD). Subsequently, other mutations in the alpha-synuclein gene have been identified in familial PD. The aggregated proteinaceous inclusions called Lewy bodies found in PD and cortical Lewy body dementia (LBD) were discovered to be predominantly alpha-synuclein. Aberrant aggregation of alpha-synuclein has been detected in an increasing number of neurodegenerative diseases, collectively known as synucleopathies. Alpha-synuclein exists physiologically in both soluble and membrane-bound states, in unstructured and alpha-helical conformations, respectively. The physiological function of alpha-synuclein appears to require its translocation between these subcellular compartments and interconversion between the 2 conformations. Abnormal processing of alpha-synuclein is predicted to lead to pathological changes in its binding properties and function.

CrossReactivity :

This antibody is known to react with alpha synuclein of human, mouse, rat and other rodents.

ALTnames :

Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP

Immunogen :

A synthetic peptide (CMPVDPDNEAYEMPSEE) of human alpha synuclein protein (aa: 116-131) conjugated to diphtheria toxoid has been used as the immunogen.

Specificity :

This antiserum specifically detects alpha synuclein.

Packaging :

Small box at room temperature

Application Summary :

IHC, WB, ELISA. Recommended to be used at a concentration of 1µg/ml for immunohistochemistry (Paraffin sections); 0.5 µg/ml for WB and immunoblot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Storage :

After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Product References :

1. Kuo Y.M. et al. (2010) Extensive enteric nervous system abnormalities in mice transgenic for artificial chromosomes containing Parkinson disease-associated alpha-synuclein gene mutations precede central nervous system changes. Hum Mol Genet. May 1;19(9):1633-50 . 2. Pelkonen A. et al. (2010) Stimulated dopamine overflow and alpha-synuclein expression in the nucleus accumbens core distinguish rats bred for differential ethanol preference. J Neurochem. 2010 Aug;114(4):1168-76. . 3. Alves et al. (2008) Striatal and nigral pathology in a lentiviral rat model of Machado-Joseph disease Hum Mol Genet. 2008 Jul 15;17(14):2071-83. .

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