This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Biosensis
product type :
antibody
product name :
Rabbit antibody to beta NGF: affinity purified
catalog :
R-174-20
quantity :
20 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry
product information
Catalog Number :
R-174-20
Product Name :
Rabbit antibody to beta NGF: affinity purified
Host Species :
Rabbit
Clonality :
Polyclonal
Product Type :
Purified
Size :
20 µg
list of Pubmed id :
18692128, 16899765
Product Description :
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
CrossReactivity :
This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
ALTnames :
Beta-nerve growth factor; Ngfb
Immunogen :
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Specificity :
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown.
Packaging :
Small box at room temperature
Application Summary :
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/ml is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/ml (ED50). This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Storage :
After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Product References :
1. Zhang H.T. et al (2008) Temporal changes in the level of neurotrophins in the spinal cord and associated precentral gyrus following spinal hemisection in adult Rhesus monkeys J Chem Neuroanat. 2008 Dec;36(3-4):138-43. . 2. Zhang H.T. et al (2007) Immunohistochemical distribution of NGF, BDNF, NT-3, and NT-4 in adult rhesus monkey brain J Histochem Cytochem. 2007 Jan;55(1):1-19. .
products id :
161
company information
Biosensis
39 Winwood Street, Thebarton, South Australia, 5031
info@biosensis.com
http://www.biosensis.com
61 8 83527711
headquarters: Australia
Biosensis specializes in antibodies, proteins, ELISA kits and Staining kits for Neuroscience, with particular emphasis on neurotrophins and neurotrophin receptors for which Biosensis has been the worldwide leader and OEM supplier for nearly 30 years. In addition to neurotrophins, our neuroscience portfolio includes products for research in neurodegenerative diseases, neurodevelopment, and neuro-metabolism. Areas of focus include Alzheimer's, Parkinson's and ALS diseases, as well as autophagy and metabolic and stress disorders including metabolic syndrome research, obesity, and neuroimmunology and inflammation.