This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Biosensis
product type :
antibody
product name :
Rabbit antibody to native DBH: whole serum
catalog :
R-108-100
quantity :
100 µl
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, bovine
application :
western blot, immunohistochemistry, immunohistochemistry - frozen section
product information
Catalog Number :
R-108-100
Product Name :
Rabbit antibody to native DBH: whole serum
Host Species :
Rabbit
Clonality :
Polyclonal
Product Type :
Whole Serum
Size :
100 µl
list of Pubmed id :
15147308, 15160400
Product Description :
DBH is an oxireductase belonging to the copper type II ascorbate-dependent monooxygenase family. DBH exists as a homotetramer composed of two non-covalently bound disulfide-linked dimers. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It binds 2 copper ions and 1 PQQ per subunit . Depending on the presence of a signal peptide, DBH can exist in both soluble and membrane-bound forms.
CrossReactivity :
This antibody is known to react with bovine, human, guinea pig and rat DBH.
ALTnames :
Dopamine beta-hydroxylase; Dopamine beta-monooxygenase; Soluble dopamine beta-hydroxylase;
Immunogen :
Native enzyme prepared from Bovine adrenal glands. Purity was greater than 95% by PAGE.
Specificity :
This antiserum has been extensively characterised in several laboratories. It reacts with a single band on western blot of bovine adrenal homogenates and stains only cells known to contain DBH, such as sympathetic neurons, adrenal medullary cells and central adrenergic neurons. Staining is abolished by preincubation with the enzyme.
Packaging :
Small box at room temperature
Application Summary :
IHC (frozen), Western Blot, radioimmunoassay and inhibition of enzymatic activity. Recommended to be used at a dilution of 1:500 to 1: 2000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Storage :
After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Product References :
Bowolaksono A. (2012) Molecular Regulation of Noradrenaline in Bovine Corpus Luteum. HAYATI Journal of Biosciences June 2012 Vol. 19 No. 2, p 81-87. Blanco-Centurion C. et al (2004) Effects of hypocretin2-saporin and antidopamine-beta-hydroxylase-saporin neurotoxic lesions of the dorsolateral pons on sleep and muscle tone. Eur J Neurosci. 2004 May;19(10):2741-52. . Ohshita N. et al (2004) Characteristics of the trigeminal depressor response in cats. J Neurosci Res. 2004 Jun 15;76(6):891-901. .
products id :
183
company information
Biosensis
39 Winwood Street, Thebarton, South Australia, 5031
info@biosensis.com
http://www.biosensis.com
61 8 83527711
headquarters: Australia
Biosensis specializes in antibodies, proteins, ELISA kits and Staining kits for Neuroscience, with particular emphasis on neurotrophins and neurotrophin receptors for which Biosensis has been the worldwide leader and OEM supplier for nearly 30 years. In addition to neurotrophins, our neuroscience portfolio includes products for research in neurodegenerative diseases, neurodevelopment, and neuro-metabolism. Areas of focus include Alzheimer's, Parkinson's and ALS diseases, as well as autophagy and metabolic and stress disorders including metabolic syndrome research, obesity, and neuroimmunology and inflammation.