This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Biosensis
product type :
antibody
product name :
Rabbit antibody to beta NGF: IgG
catalog :
R-093-500
quantity :
500 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry
product information
Catalog Number :
R-093-500
Product Name :
Rabbit antibody to beta NGF: IgG
Host Species :
Rabbit
Clonality :
Polyclonal
Product Type :
Purified
Size :
500 µg
list of Pubmed id :
19773628, 17316840
Product Description :
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
CrossReactivity :
This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
ALTnames :
Beta-nerve growth factor
Immunogen :
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Specificity :
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown.
Packaging :
Small box at room temperature
Application Summary :
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/ml is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/ml (ED50). This antibody was tested on cultured sensory neurons supported by 100ng/ml of purified mouse beta NGF. Be advised that 2ug/ml will neutralize 100ng/ml of mouse NGF. The higher 10ug/ml is only recommended if the concentration of NGF being used is higher than 100ng/ml such as the 200 or 500ng/ml that is occasionally used in some culture systems. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Storage :
After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Product References :
1. Laurina Z. et al (2009) Growth factors/cytokines/defensins and apoptosis in periodontal pathologies. Stomatologija. 2009;11(2):48-54. . 2. Lee H.W. et al (2007) Expression of nerve growth factor is upregulated in the rat thymic epithelial cells during thymus regeneration following acute thymic involution. Regul Pept. 2007 Jun 7;141(1-3):86-95 .
products id :
160
company information
Biosensis
39 Winwood Street, Thebarton, South Australia, 5031
info@biosensis.com
http://www.biosensis.com
61 8 83527711
headquarters: Australia
Biosensis specializes in antibodies, proteins, ELISA kits and Staining kits for Neuroscience, with particular emphasis on neurotrophins and neurotrophin receptors for which Biosensis has been the worldwide leader and OEM supplier for nearly 30 years. In addition to neurotrophins, our neuroscience portfolio includes products for research in neurodegenerative diseases, neurodevelopment, and neuro-metabolism. Areas of focus include Alzheimer's, Parkinson's and ALS diseases, as well as autophagy and metabolic and stress disorders including metabolic syndrome research, obesity, and neuroimmunology and inflammation.