Rabbit antibody to BDNF (129-138): whole serum | Biosensis R-083-100 product information

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product summary

company name :

Biosensis

product type :

antibody

product name :

Rabbit antibody to BDNF (129-138): whole serum

catalog :

R-083-100

quantity :

100 µl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

immunohistochemistry

product information

Catalog Number :

R-083-100

Product Name :

Rabbit antibody to BDNF (129-138): whole serum

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Whole Serum

Size :

100 µl

list of Pubmed id :

25310564, 19969506, 19083991

Product Description :

BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.

CrossReactivity :

This antiserum is known to recognise rat, mouse and human BDNF.

ALTnames :

Brain-derived neurotrophic factor; Abrineurin; proBDNF;

Immunogen :

A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst primates.

Specificity :

Less than 0.1% cross reactivity with mouse NGF, recombinant human NT3 and NT4/5 has been recorded by dot blot analysis.

Packaging :

Small box at room temperature

Application Summary :

IHC, ELISA, WB. A working dilution of 1:1000 to 1:2000 is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Storage :

After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Product References :

. McLean NA, Popescu BF, Gordon T, Zochodne DW, Verge VM. (2014) "Delayed nerve stimulation promotes axon-protective neurofilament phosphorylation, accelerates immune cell clearance and enhances remyelination in vivo in focally demyelinated nerves." PLoS One. 2014 Oct 13;9(10):e110174 Application: WB , non-reducing, Species: Rat . Cysneiros R.M. et al (2010) Qualitative analysis of hippocampal plastic changes in rats with epilepsy supplemented with oral omega-3 fatty acids Epilepsy Behav. 2010 Jan;17(1):33-8. . Ooe N. et al (2009) Dynamic regulation of bHLH-PAS-type transcription factor NXF gene expression and neurotrophin dependent induction of the transcriptional control activity Biochem Biophys Res Commun. 2009 Jan 23;378(4):761-5. .

products id :

153