Mouse monoclonal antibody to human p75NTR [MLR2] - FITC | Biosensis M-018-100-FT product information

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product summary

company name :

Biosensis

product type :

antibody

product name :

Mouse monoclonal antibody to human p75NTR [MLR2] - FITC

catalog :

M-018-100-FT

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

FITC

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

citations: 2

Reference
Chacon P, Arevalo M, Tébar A. NGF-activated protein tyrosine phosphatase 1B mediates the phosphorylation and degradation of I-kappa-Balpha coupled to NF-kappa-B activation, thereby controlling dendrite morphology. Mol Cell Neurosci. 2010;43:384-93 pubmed publisher
Wiese S, Herrmann T, Drepper C, Jablonka S, Funk N, Klausmeyer A, et al. Isolation and enrichment of embryonic mouse motoneurons from the lumbar spinal cord of individual mouse embryos. Nat Protoc. 2010;5:31-8 pubmed publisher

product information

Catalog Number :

M-018-100-FT

Product Name :

Mouse monoclonal antibody to human p75NTR [MLR2] - FITC

Host Species :

Mouse

Clonality :

Monoclonal

Conjugation :

FITC

Product Type :

Purified

Size :

100 µg

list of Pubmed id :

20057379, 20123020

Clone :

MLR2

Isotype :

IgG2a

Product Description :

p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR is not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1.

CrossReactivity :

Reacts with human, mouse and rat. Other species have not yet been tested but it is expected that this antibody will be useful for the study of p75 in primates and other species.

ALTnames :

Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; Tumor necrosis factor receptor superfamily member 16; CD271; NGFR; TNFRSF16;

Immunogen :

Human recombinant p75

Specificity :

Specificity was demonstrated by immunohistochemistry and FACS analysis. Immunohistochemical staining of motor neurons in spinal cord following lesion of sciatic nerve and in cholinergic neurons of the basal forebrain corresponding to published literature.

Packaging :

Foam box with ice packs

Application Summary :

Immunohistochemistry, immunofluorescence, Western Blot (non-denaturing gels), flow cytometry (Anti NGF receptor p75 is CD271), anti-NGF receptor p75 dynamics, retrograde transport studies, study of intracellular trafficking (see IHC image attached).Suggested working dilutions:For immunohistochemistry a concentration of 1-2 µg/ml is recommended. This antibody is not recommended for denaturing WB applications. For FACS a concentration of 20 µg/ml is recommended and for 1 site ELISA at least a 1 in 5000 dilution.This antibody does not block Neurotrophin binding. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Storage :

The antibody conjugate can be stored at 4ºC for up to 4 months with the addition of appropriate antibacterial agent.

Product References :

1. Wiese S, Herrmann T, Drepper C, Jablonka S, Funk N, Klausmeyer A, Rogers ML, Rush RA & Sendtner M (2009) Isolation and enrichment of embryonic mouse motoneurons from the lumbar spinal cord of individual mouse embryos. Nat Protoc. 2010;5(1):31-8. . 2. ChacÃ³n PJ, ArÃ©valo MA, TÃ©bar AR. (2010) NGF-activated protein tyrosine phosphatase 1B mediates the phosphorylation and degradation of I-kappa-Balpha coupled to NF-kappa-B activation, thereby controlling dendrite morphology. Mol Cell Neurosci. 2010 Apr;43(4):384-93. .

products id :

260