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product summary
company name :
Biosensis
product type :
antibody
product name :
Mouse monoclonal antibody to human p75NTR [MLR2]: IgG2a
catalog :
M-009-100
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human, mouse, rat
citations: 9
Reference
Yadav P, Selvaraj B, Bender F, Behringer M, Moradi M, Sivadasan R, et al. Neurofilament depletion improves microtubule dynamics via modulation of Stat3/stathmin signaling. Acta Neuropathol. 2016;132:93-110 pubmed publisher
Klausmeyer A, Stern D, Wiese S. Isolation and culture of spinal cord motor neurons. Curr Protoc Cell Biol. 2015;66:1.9.1-10 pubmed publisher
Wiszniak S, Kabbara S, Lumb R, Scherer M, Secker G, Harvey N, et al. The ubiquitin ligase Nedd4 regulates craniofacial development by promoting cranial neural crest cell survival and stem-cell like properties. Dev Biol. 2013;383:186-200 pubmed publisher
Panni P, Ferguson I, Beacham I, Mackay Sim A, Ekberg J, St John J. Phagocytosis of bacteria by olfactory ensheathing cells and Schwann cells. Neurosci Lett. 2013;539:65-70 pubmed publisher
Selvaraj B, Frank N, Bender F, Asan E, Sendtner M. Local axonal function of STAT3 rescues axon degeneration in the pmn model of motoneuron disease. J Cell Biol. 2012;199:437-51 pubmed publisher
Zhang C, Helmsing S, Zagrebelsky M, Schirrmann T, Marschall A, Schüngel M, et al. Suppression of p75 neurotrophin receptor surface expression with intrabodies influences Bcl-xL mRNA expression and neurite outgrowth in PC12 cells. PLoS ONE. 2012;7:e30684 pubmed publisher
Rogers M, Bailey S, Matusica D, Nicholson I, Muyderman H, Pagadala P, et al. ProNGF mediates death of Natural Killer cells through activation of the p75NTR-sortilin complex. J Neuroimmunol. 2010;226:93-103 pubmed publisher
Chacon P, Arevalo M, Tébar A. NGF-activated protein tyrosine phosphatase 1B mediates the phosphorylation and degradation of I-kappa-Balpha coupled to NF-kappa-B activation, thereby controlling dendrite morphology. Mol Cell Neurosci. 2010;43:384-93 pubmed publisher
Wiese S, Herrmann T, Drepper C, Jablonka S, Funk N, Klausmeyer A, et al. Isolation and enrichment of embryonic mouse motoneurons from the lumbar spinal cord of individual mouse embryos. Nat Protoc. 2010;5:31-8 pubmed publisher
product information
Catalog Number :
M-009-100
Product Name :
Mouse monoclonal antibody to human p75NTR [MLR2]: IgG2a
Host Species :
Mouse
Clonality :
Monoclonal
Product Type :
Purified
Size :
100 µg
list of Pubmed id :
24080509, 23415759, 22292018, 20057379, 20123020, 23109669, 20547427
Clone :
MLR2
Isotype :
IgG2a
Product Description :
p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR is not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3.
CrossReactivity :
This antibody is known to react with human, mouse, rat and guinea-pig p75NTR protein.
ALTnames :
Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR
Immunogen :
This antibody was raised against chimeric recombinant human p75 protein coupled to an Fc region of human immunoglobulin.
Specificity :
Specificity has been confirmed using a number of techniques as described in the reference by Rogers et al (2006). The antibody recognizes extracellular p75 in native configurations.
Packaging :
Small box at room temperature
Application Summary :
IH (fresh, acetone fixed sections only, epitope is fixation sensitive), Not suitable in paraffin embedded tissues. Western Blot (non-denaturing conditions only) and Immunopanning. A concentration of 1-5 µg/ml is recommended for immunohistochemistry, immunopanning and WB. This antibody is not recommended for denaturing WB applications . A concentration of 20 µg/ml is recommended for immunofluorescence and FACS. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Storage :
After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Product References :
Wiszniak S, Kabbara S, Lumb R, Scherer M, Secker G, Harvey N, Kumar S, Schwarz Q. (2013) "The ubiquitin ligase Nedd4 regulates cranofacial development by promoting cranial neural crest cell survival and stem-cell like properties" Dev Biol. 2013 Nov 15.;383(2):186-200. doi: 10.1016/j.ydbio.2013.09.024. Application: Immunohistochemistry. Species: Mouse. Panni P, Ferguson IA, Beacham I, Mackay-Sim A, Ekberg JA, John JA. (2013) "Phagocytosis of bacteria by olfactory ensheathing cells and Schwann cells" Neurosci Lett. 2013 Feb 13. pii: S0304-3940(13)00109-2. Application: Immunopanning, species: Mouse. Zhang C. et al (2012) Suppression of p75 neurotrophin receptor surface expression with intrabodies influences Bcl-xL mRNA expression and neurite outgrowth in PC12 cells PLoS One. 2012;7(1) . Wiese S, Herrmann T, Drepper C, Jablonka S, Funk N, Klausmeyer A, Rogers ML, Rush RA & Sendtner M (2009) Isolation and enrichment of embryonic mouse motoneurons from the lumbar spinal cord of individual mouse embryos. Nat Protoc. 2010;5(1):31-8. . Chacón PJ, Arévalo MA, Tébar AR. (2010) NGF-activated protein tyrosine phosphatase 1B mediates the phosphorylation and degradation of I-kappa-Balpha coupled to NF-kappa-B activation, thereby controlling dendrite morphology. Mol Cell Neurosci. 2010 Apr;43(4):384-93. . Selvaraj BT et al (2012) Local axonal function of STAT3 rescues axon degeneration in the pmn model of motoneuron disease. J Cell Biol. 2012 Oct 29;199(3):437-51. . Rogers ML et al (2010) ProNGF mediates death of Natural Killer cells through activation of the p75NTR-sortilin complex. J Neuroimmunol. 2010 Sep 14;226(1-2):93-103. .
products id :
159
company information
Biosensis
39 Winwood Street, Thebarton, South Australia, 5031
info@biosensis.com
http://www.biosensis.com
61 8 83527711
headquarters: Australia
Biosensis specializes in antibodies, proteins, ELISA kits and Staining kits for Neuroscience, with particular emphasis on neurotrophins and neurotrophin receptors for which Biosensis has been the worldwide leader and OEM supplier for nearly 30 years. In addition to neurotrophins, our neuroscience portfolio includes products for research in neurodegenerative diseases, neurodevelopment, and neuro-metabolism. Areas of focus include Alzheimer's, Parkinson's and ALS diseases, as well as autophagy and metabolic and stress disorders including metabolic syndrome research, obesity, and neuroimmunology and inflammation.