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product summary
company name :
Biosensis
product type :
ELISA/assay
product name :
proNGF Rapid ELISA Kit: Human (2 plates)
catalog :
BEK-2226-2P
quantity :
2 Plate Kit (2 x 96
product information
Catalog Number :
BEK-2226-2P
Product Name :
proNGF Rapid ELISA Kit: Human (2 plates)
Size :
2 Plate Kit (2 x 96 well plate)2 Plate Kit (2 x 96 well plate)2 Plate Kit (2 x 96 well plate)2 Plate Kit (2 x 96 well plate)2 Plate Kit (2 x 96 well plate)2 Plate Kit (2 x 96 well plate)
Product Description :
The Biosensis proNGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of full-length proNGF protein in less than 4 hours. This kit consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3€™,5,5€™-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. This proNGF ELISA kit contains a recombinant human proNGF standard expressed in E.coli. This ELISA kit does not cross-react with the mouse form of proNGF, and due to sequence homology of mouse and rat proNGF is not expected to detect rat proNGF. The antibodies used in this ELISA kit bind epitopes within the pro-domain of the protein and therefore recognize proNGF, the pro-domain peptide, but do not cross-react with mature NGF! The purpose of this kit is the in vitro quantitative determination of proNGF in cell culture supernatants and human serum. This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
CrossReactivity :
Does not cross-react with recombinant human NGF and proBDNF tested at 25 ng/mL.
ALTnames :
pro-brain nerve growth factor; proNGF; NGF
Immunogen :
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
Specificity :
Human. Does not react with mouse proNGF, and is not expected to detect rat proNGF.
Packaging :
Foam box with ice packs
Storage :
Store at 4°C.
products id :
1503
company information
Biosensis
39 Winwood Street, Thebarton, South Australia, 5031
info@biosensis.com
http://www.biosensis.com
61 8 83527711
headquarters: Australia
Biosensis specializes in antibodies, proteins, ELISA kits and Staining kits for Neuroscience, with particular emphasis on neurotrophins and neurotrophin receptors for which Biosensis has been the worldwide leader and OEM supplier for nearly 30 years. In addition to neurotrophins, our neuroscience portfolio includes products for research in neurodegenerative diseases, neurodevelopment, and neuro-metabolism. Areas of focus include Alzheimer's, Parkinson's and ALS diseases, as well as autophagy and metabolic and stress disorders including metabolic syndrome research, obesity, and neuroimmunology and inflammation.