ELISA/assay

GDNF Rapid ELISA Kit: Human (1 plate)

BEK-2222-1P

1 Plate Kit (1 x 96

GDNF Rapid ELISA Kit: Human (1 plate)

1 Plate Kit (1 x 96 well plate)1 Plate Kit (1 x 96 well plate)1 Plate Kit (1 x 96 well plate)1 Plate Kit (1 x 96 well plate)1 Plate Kit (1 x 96 well plate)1 Plate Kit (1 x 96 well plate)

The Biosensis GDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours. This kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3€™,5,5€™- tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. This GDNF ELISA kit employs a recombinant human GDNF standard obtained from the National Institute for Biological Standards and Control (NIBSC) and is therefore designed to accurately measure the human form of GDNF. Note that the antibodies used in this kit cross-react with rat and mouse GDNF. This kit can be used for in vitro quantitative determination of GDNF in samples such as serum, plasma (EDTA and citrate), cell culture supernatants and cell lysates. Please see the sample preparation section in the attached protocol for further details.This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

No cross-reactivity was observed for the following proteins tested at 25 ng/mL: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.

Glial cell line-derived neurotrophic factor; Astrocyte-derived trophic factor; ATF; GDNF

GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.

Human. The antibodies used in this kit detect mouse and rat GDNF.

Foam box with ice packs

Store at 4°C.

1507