ELISA/assay

NGFR/p75ECD Rapid ELISA Kit: Human (1 Plate)

BEK-2219-1P

1 Plate Kit (1 x 96

NGFR/p75ECD Rapid ELISA Kit: Human (1 Plate)

1 Plate Kit (1 x 96 well plate)1 Plate Kit (1 x 96 well plate)1 Plate Kit (1 x 96 well plate)1 Plate Kit (1 x 96 well plate)1 Plate Kit (1 x 96 well plate)1 Plate Kit (1 x 96 well plate)

The Biosensis NGFR/p75 ECD Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of human p75 neurotrophin receptor in less than 4 hours. This kit consists of a pre-coated mouse monoclonal anti-p75 ECD capture antibody, a goat anti-p75 ECD detection antibody and a horseradish peroxidase (HRP)-conjugated anti-goat antibody. The addition of a substrate (3,3€™,5,5€™-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75 ECD present in samples and protein standards. This NGFR/p75 ECD ELISA kit employs an in-house recombinant human p75 ECD -Fc chimera as standard that has been validated against an externally available p75 ECD -Fc protein. While there is a current lack of a commercially available, true human p75 ECD standard, this ELISA kit allows quantification of human p75 ECD as p75 ECD -Fc human equivalents. Please note that the antibodies used in this ELISA cross-react with mouse p75 ECD . The purpose of this kit is the i n vitro quantitative determination of human p75 ECD in samples such as cell culture supernatants and urine. This kit has not been tested for other applications. Note: For research use only. Not for diagnostic and clinical purposes.

The antibodies used in this ELISA kit are known to cross-react with mouse p75ECD protein.

Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; Tumor necrosis factor receptor superfamily member 16; CD271; NGFR; TNFRSF16.

The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.

Human

Foam box with ice packs

Store at 2-8°C.

1299